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Predicted conserved domains of Pep4, Prb1, PaPRO1p, and PaPRO2p.
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Predicted signal peptides of PaPRO1p and PaPRO2p.
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Phylogenetic tree of aspartic proteases of Saccharomyces cerevisiae and Candida...
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Expression levels of the three candidate genes of PEP4 .
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Alignments of amino acid sequences of three candidates with Pep4.
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Plasmids expressing Pa PRO1 and Pa PRO2 used in complement experiments.
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Intracellular protease activity of ΔPa PRO1 , ΔPa PRO2 , and wild-type.
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Evaluation of protease activity in culture supernatant of ΔPa PRO1 , ΔPa PRO2 ,...
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Predicted conserved domains of candidate#1, #2, and #3.
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Effect of ΔPa PRO2 on degradation and PaE activity during large-scale culture wi...
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Complementation of gene deletion in ΔPa PRO1 and ΔPa PRO2 mutants.
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PaE production in ΔPa PRO1 and ΔPa PRO2 mutants.
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Conserved catalytic motifs in PaPRO1p and PaPRO2p.
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Constructing deletion mutants of Pa PRO1 and Pa PRO2 .
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List of plasmids and strains.
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Primer sets to construct each strain.
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Sequences of the primers used.
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Still Image
Author
Hideaki Koike (410323)
20
Hiroko Kitamoto (6076307)
20
Kazunori Ushimaru (3689119)
20
Natsuki Omae (10318504)
20
Tomotake Morita (526538)
20
Yuka Sameshima-Yamashita (6292238)
20
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Topic
3- L jar fermenter
20
48 h incubation
20
B orthologous genes
20
Biochemistry
20
Biological Sciences not elsewhere classified
20
Biophysics
20
Cell Biology
20
Chemical Sciences not elsewhere classified
20
Developmental Biology
20
GB
20
Genetics
Infectious Diseases
20
Microbiology
20
Molecular Biology
20
PaE
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Science Policy
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XG 8
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XG 8 control
XG 8 Δ Pa
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degradation fragments
20
orthologous genes encoding proteases
20
plastic-degrading enzyme
20
strain
vacuolar protease function
yeast Pseudozyma antarctica
20
Δ Pa
20
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Unknown
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Aldrich
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St. Louis
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unknown
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Open Polar Keyword
Antarc*
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Antarctica
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