Effect of ΔPa PRO2 on degradation and PaE activity during large-scale culture with XG8.

• Main Authors: Natsuki Omae (10318504), Yuka Sameshima-Yamashita (6292238), Kazunori Ushimaru (3689119), Hideaki Koike (410323), Hiroko Kitamoto (6076307), Tomotake Morita (526538)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Biophysics; Biochemistry; Microbiology; Cell Biology; Genetics; Molecular Biology; Developmental Biology; Science Policy; Infectious Diseases; Biological Sciences not elsewhere classified; Chemical Sciences not elsewhere classified; vacuolar protease function; yeast Pseudozyma antarctica; GB; plastic-degrading enzyme; 48 h incubation; orthologous genes encoding proteases; 3- L jar fermenter; XG 8; XG 8 Δ Pa; strain; XG 8 control; B orthologous genes; PaE; degradation fragments; Δ Pa; Antarc*; Antarctica
• Online Access: https://doi.org/10.1371/journal.pone.0247462.g004

(A) Mutant strain ΔPa PRO2 and its parent strain, XG8, were cultured in a jar fermenter. Extracellularly secreted PaE was analyzed by Coomassie Brilliant Blue stain. Black closed triangle, full-length PaE; open triangles, degradation fragments of PaE in strain GB-4(0). (B) Dry cell weight and activity (U/mL) of PaE in strains XG8 and ΔPa PRO2 -XG8 based on the result of 6 measurements. The standard deviation was indicated by error bar. (C) The effect of filtration removing the cells from culture on PaE activity (U/mL) in strains XG8 and ΔPa PRO2 -XG8 based on the result of 6 measurements. The standard deviation was indicated by error bar.