Phylogenetic tree of aspartic proteases of Saccharomyces cerevisiae and Candida albicans , a PEP4 ortholog of Ustilago maydis , and the three candidate genes for PEP4 of Pseudozyma antarctica was predicted neighbor-joining method [31].

• Main Authors: Natsuki Omae (10318504), Yuka Sameshima-Yamashita (6292238), Kazunori Ushimaru (3689119), Hideaki Koike (410323), Hiroko Kitamoto (6076307), Tomotake Morita (526538)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Biophysics; Biochemistry; Microbiology; Cell Biology; Genetics; Molecular Biology; Developmental Biology; Science Policy; Infectious Diseases; Biological Sciences not elsewhere classified; Chemical Sciences not elsewhere classified; vacuolar protease function; yeast Pseudozyma antarctica; GB; plastic-degrading enzyme; 48 h incubation; orthologous genes encoding proteases; 3- L jar fermenter; XG 8; XG 8 Δ Pa; strain; XG 8 control; B orthologous genes; PaE; degradation fragments; Δ Pa; Antarc*; Antarctica
• Online Access: https://doi.org/10.1371/journal.pone.0247462.s008

The evolutionary distance was calculated with Poisson correction method [ 32 ] and the tree was shown in scale. This analysis was performed in MEGA X [ 33 , 34 ]. (TIF)