Complementation of gene deletion in ΔPa PRO1 and ΔPa PRO2 mutants.
Secreted PaE in mutant strains (ΔPa PRO1 or ΔPa PRO2 ) bearing the plasmids pEmp-Pa PRO1 , pEmp-Pa PRO2 or pEmp, and cultured for 8 days, were detected by immunoblotting with anti-PaE antibody. PC, purified PaE as a positive control; black triangle, full-length PaE; gray triangles, degraded fragment...
Main Authors: | , , , , , |
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Format: | Still Image |
Language: | unknown |
Published: |
2021
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Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pone.0247462.g003 |
Summary: | Secreted PaE in mutant strains (ΔPa PRO1 or ΔPa PRO2 ) bearing the plasmids pEmp-Pa PRO1 , pEmp-Pa PRO2 or pEmp, and cultured for 8 days, were detected by immunoblotting with anti-PaE antibody. PC, purified PaE as a positive control; black triangle, full-length PaE; gray triangles, degraded fragment of PaE in strain GB-4(0). |
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