Complementation of gene deletion in ΔPa PRO1 and ΔPa PRO2 mutants.

Secreted PaE in mutant strains (ΔPa PRO1 or ΔPa PRO2 ) bearing the plasmids pEmp-Pa PRO1 , pEmp-Pa PRO2 or pEmp, and cultured for 8 days, were detected by immunoblotting with anti-PaE antibody. PC, purified PaE as a positive control; black triangle, full-length PaE; gray triangles, degraded fragment...

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Bibliographic Details
Main Authors: Natsuki Omae (10318504), Yuka Sameshima-Yamashita (6292238), Kazunori Ushimaru (3689119), Hideaki Koike (410323), Hiroko Kitamoto (6076307), Tomotake Morita (526538)
Format: Still Image
Language:unknown
Published: 2021
Subjects:
GB
PaE
Online Access:https://doi.org/10.1371/journal.pone.0247462.g003
Description
Summary:Secreted PaE in mutant strains (ΔPa PRO1 or ΔPa PRO2 ) bearing the plasmids pEmp-Pa PRO1 , pEmp-Pa PRO2 or pEmp, and cultured for 8 days, were detected by immunoblotting with anti-PaE antibody. PC, purified PaE as a positive control; black triangle, full-length PaE; gray triangles, degraded fragment of PaE in strain GB-4(0).