Constructing deletion mutants of Pa PRO1 and Pa PRO2 .

Disruptive fragments for Pa PRO1 and Pa PRO2 are shown in (A) and (B), respectively. Numbers and arrows indicate primers. Descriptions of primers and amplicons are provided in S1 – S3 Tables. Deletion of the Pa PRO1 (C) or Pa PRO2 (D) gene was verified by PCR during positive and negative screening....

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Bibliographic Details
Main Authors: Natsuki Omae (10318504), Yuka Sameshima-Yamashita (6292238), Kazunori Ushimaru (3689119), Hideaki Koike (410323), Hiroko Kitamoto (6076307), Tomotake Morita (526538)
Format: Still Image
Language:unknown
Published: 2021
Subjects:
Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
Antarc*
Antarctica
Online Access:https://doi.org/10.1371/journal.pone.0247462.s004
Description
Summary:Disruptive fragments for Pa PRO1 and Pa PRO2 are shown in (A) and (B), respectively. Numbers and arrows indicate primers. Descriptions of primers and amplicons are provided in S1 – S3 Tables. Deletion of the Pa PRO1 (C) or Pa PRO2 (D) gene was verified by PCR during positive and negative screening. (TIF)

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