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Predicted conserved domains of Pep4, Prb1, PaPRO1p, and PaPRO2p.
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Predicted signal peptides of PaPRO1p and PaPRO2p.
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Phylogenetic tree of aspartic proteases of Saccharomyces cerevisiae and Candida...
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Expression levels of the three candidate genes of PEP4 .
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Alignments of amino acid sequences of three candidates with Pep4.
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Plasmids expressing Pa PRO1 and Pa PRO2 used in complement experiments.
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S3 Raw image -
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Intracellular protease activity of ΔPa PRO1 , ΔPa PRO2 , and wild-type.
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Evaluation of protease activity in culture supernatant of ΔPa PRO1 , ΔPa PRO2 ,...
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Predicted conserved domains of candidate#1, #2, and #3.
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Predicted orthologous genes of PEP4 and PRB1 in GB-4(0).
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Effect of ΔPa PRO2 on degradation and PaE activity during large-scale culture wi...
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Complementation of gene deletion in ΔPa PRO1 and ΔPa PRO2 mutants.
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PaE production in ΔPa PRO1 and ΔPa PRO2 mutants.
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Conserved catalytic motifs in PaPRO1p and PaPRO2p.
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Constructing deletion mutants of Pa PRO1 and Pa PRO2 .
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List of plasmids and strains.
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Primer sets to construct each strain.
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3- L jar fermenter
48 h incubation
B orthologous genes
Biochemistry
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XG 8
XG 8 control
XG 8 Δ Pa
degradation fragments
orthologous genes encoding proteases
plastic-degrading enzyme
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vacuolar protease function
yeast Pseudozyma antarctica
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