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Concentration of PaE by combination of ammonium sulfate precipitation and ethano...
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Concentration of PaE by ethanol precipitation.
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Survival of Pseudozyma antarctica cells after ethanol treatment.
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Fig 2 -
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Concentration of PaE by precipitation with ammonium sulfate and ethanol.
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SDS-PAGE analysis of the purified PaE.
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S2 Raw image -
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Predicted conserved domains of Pep4, Prb1, PaPRO1p, and PaPRO2p.
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Predicted signal peptides of PaPRO1p and PaPRO2p.
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Phylogenetic tree of aspartic proteases of Saccharomyces cerevisiae and Candida...
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Expression levels of the three candidate genes of PEP4 .
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Alignments of amino acid sequences of three candidates with Pep4.
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Plasmids expressing Pa PRO1 and Pa PRO2 used in complement experiments.
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Intracellular protease activity of ΔPa PRO1 , ΔPa PRO2 , and wild-type.
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Evaluation of protease activity in culture supernatant of ΔPa PRO1 , ΔPa PRO2 ,...
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Predicted conserved domains of candidate#1, #2, and #3.
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Related Subjects
Biochemistry
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
Microbiology
PaE
Science Policy
3- L jar fermenter
48 h incubation
B orthologous genes
Biophysics
Cell Biology
Developmental Biology
GB
Genetics
Infectious Diseases
Molecular Biology
XG 8
XG 8 control
XG 8 Δ Pa
degradation fragments
orthologous genes encoding proteases
plastic-degrading enzyme
strain
vacuolar protease function
yeast Pseudozyma antarctica
Δ Pa
Astronomical and Space Sciences not elsewhere classified
Biotechnology
Ecology
Environmental Sciences not elsewhere classified
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