| Summary: | (a) Temporal changes in PaE activity in culture filtrates containing various amounts of ethanol. Ethanol was added to a final concentration of 0%, 10%, 25%, 50%, 75%, or 90% (v/v), and then the enzyme solution was stored at ambient temperature (RT) for up to 206 days. Relative activities are expressed as a percentage of the initial (0 day) activity of the control culture filtrate (containing 0% ethanol). Data are means ± standard deviation (SD) ( n = 3). (b) Residual PaE activity in the solution of partially purified PaE containing 20% ethanol. Partially purified PaE obtained by preparation with the combination of ammonium sulfate precipitation and ethanol precipitation was dissolved in 20% ethanol and stored at 4°C or RT for 230 days. Relative activities are expressed as a percentage of the initial (0 day) activity. Data are presented as the mean ± SD ( n = 3).
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