Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua)

Publisher's version (útgefin grein) Atlantic cod trypsin ZT is biochemically characterized for the first time in this report in comparison to a group I trypsin (cod trypsin I). To our knowledge, trypsin ZT is the first thoroughly characterized group III trypsin. A more detailed understanding of...

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Bibliographic Details
Published in:International Journal of Biological Macromolecules
Main Authors: Sandholt, Gunnar Birgir, Stefánsson, Bjarki, Scheving, Reynir, Guðmundsdóttir Ágústa
Other Authors: Matvæla- og næringarfræðideild (HÍ), Faculty of Food Science and Nutrition (UI), Heilbrigðisvísindasvið (HÍ), School of Health Sciences (UI), Háskóli Íslands, University of Iceland
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier BV 2019
Subjects:
Online Access:https://hdl.handle.net/20.500.11815/1792
https://doi.org/10.1016/j.ijbiomac.2018.12.099
Description
Summary:Publisher's version (útgefin grein) Atlantic cod trypsin ZT is biochemically characterized for the first time in this report in comparison to a group I trypsin (cod trypsin I). To our knowledge, trypsin ZT is the first thoroughly characterized group III trypsin. A more detailed understanding of trypsin ZT biochemistry may give insight into its physiological role as well as its potential use within the biotechnology sector. Stability is an important factor when it comes to practical applications of enzymes. Compared to trypsin I, trypsin ZT shows differences in pH and heat stability, sensitivity to inhibitors and sub-site substrate specificity as shown by multiplex substrate profiling analysis. Based on the analysis, trypsin ZT cleaved at arginine and lysine as other trypsins. Furthermore, trypsin ZT is better than trypsin I in cleaving peptides containing several consecutive positively charged residues. Lysine- and arginine-rich amino acid sequences are frequently found in human viral proteins. Thus, trypsin ZT may be effective in inactivating human and fish viruses implying a possible role for the enzyme in the natural defence of Atlantic cod. The results from this study can lead to multiple practical applications of trypsin ZT. This work was supported by the AVS R&D Fund of Ministry of Fisheries and Agriculture in Iceland [grant reference number: R15 046-15 , R069-08 , R11 028-11 and R14 044-14 ]; and Technology Development Fund (The Icelandic Centre for Research ) [grant reference number: 120852-0611 and 131804-0611 ]. The funding source had no involvement in study design; in the collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the article for publication. Peer Reviewed