Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion
Summary Bioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and after in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellula...
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Faculty of Food Technology and Biotechnology, University of Zagreb
2017
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fthrcak:oai:hrcak.srce.hr:192536 2023-05-15T16:19:15+02:00 Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion Jamnik, Polona Istenič, Katja Koštomaj, Tatjana Wulff, Tune Geirsdóttir, Margrét Almgren, Annette Jónsdóttir, Rósa Kristinsson, Hordur G. Undeland, Ingrid 2017-12-29 application/pdf https://hrcak.srce.hr/192536 https://hrcak.srce.hr/file/284070 eng eng Faculty of Food Technology and Biotechnology, University of Zagreb info:eu-repo/semantics/altIdentifier/doi/10.17113/ftb.55.03.17.5117 https://hrcak.srce.hr/192536 info:eu-repo/semantics/openAccess The contents of the Food Technology and Biotechnology can be used for personal, educational or scientific purposes, provided that the credit is given to the Journal (under Creative Commons licence CC BY-NC 4.0). Annual subscription price for all countries (except Croatia) is 40 euro for citizens and 100 euro for organizations and institutions. Discount is offered to the members of Croatian Society of Biotechnology and Slovenian Microbiological Society. For subscription please contact the Editorial Office at the E-mail address: ftb@pbf.hr CC-BY-NC Food Technology and Biotechnology ISSN 1330-9862 (Print) ISSN 1334-2606 (Online) Volume 55 Issue 4 protein hydrolysates;cod;chicken;in vitro gastrointestinal digestion;yeast;proteomics text info:eu-repo/semantics/other info:eu-repo/semantics/publishedVersion 2017 fthrcak https://doi.org/10.17113/ftb.55.03.17.5117 2018-01-17T23:54:48Z Summary Bioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and after in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellular exposure to simulate digestion conditions in the human body and therefore investigate the role of modulations in the GI tract on the cell response. The effect of digested and undigested hydrolysates on intracellular oxidation, cellular metabolic energy and proteome level was investigated. No difference in the effect on intracellular oxidation activity was obtained between cod and chicken hydrolysates, while higher affect on intracellular oxidation was provided by digested hydrolysates, with relative values of intracellular oxidation of cod of (70.2±0.8) and chicken of (74.5±1.4) % than by undigested ones, where values of cod and chicken were (95.5±1.2) and (90.5±0.7) %, respectively. Neither species nor digestion had any effect on cellular metabolic energy. At proteome level, digested hydrolysates gave again significantly stronger responses than undigested counterparts; cod peptides here also gave somewhat stronger response than chicken peptides. The knowledge of the action of food protein hydrolysates and their digests within live cells, also at proteome level, is important for further validation of their activity in higher eukaryotes to develop new functional food ingredients, such as in this case chicken and cod muscle-derived peptides. Text Gadus morhua Hrčak - Portal of scientific journals of Croatia Food Technology and Biotechnology 55 3 |
institution |
Open Polar |
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Hrčak - Portal of scientific journals of Croatia |
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fthrcak |
language |
English |
topic |
protein hydrolysates;cod;chicken;in vitro gastrointestinal digestion;yeast;proteomics |
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protein hydrolysates;cod;chicken;in vitro gastrointestinal digestion;yeast;proteomics Jamnik, Polona Istenič, Katja Koštomaj, Tatjana Wulff, Tune Geirsdóttir, Margrét Almgren, Annette Jónsdóttir, Rósa Kristinsson, Hordur G. Undeland, Ingrid Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
topic_facet |
protein hydrolysates;cod;chicken;in vitro gastrointestinal digestion;yeast;proteomics |
description |
Summary Bioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and after in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellular exposure to simulate digestion conditions in the human body and therefore investigate the role of modulations in the GI tract on the cell response. The effect of digested and undigested hydrolysates on intracellular oxidation, cellular metabolic energy and proteome level was investigated. No difference in the effect on intracellular oxidation activity was obtained between cod and chicken hydrolysates, while higher affect on intracellular oxidation was provided by digested hydrolysates, with relative values of intracellular oxidation of cod of (70.2±0.8) and chicken of (74.5±1.4) % than by undigested ones, where values of cod and chicken were (95.5±1.2) and (90.5±0.7) %, respectively. Neither species nor digestion had any effect on cellular metabolic energy. At proteome level, digested hydrolysates gave again significantly stronger responses than undigested counterparts; cod peptides here also gave somewhat stronger response than chicken peptides. The knowledge of the action of food protein hydrolysates and their digests within live cells, also at proteome level, is important for further validation of their activity in higher eukaryotes to develop new functional food ingredients, such as in this case chicken and cod muscle-derived peptides. |
format |
Text |
author |
Jamnik, Polona Istenič, Katja Koštomaj, Tatjana Wulff, Tune Geirsdóttir, Margrét Almgren, Annette Jónsdóttir, Rósa Kristinsson, Hordur G. Undeland, Ingrid |
author_facet |
Jamnik, Polona Istenič, Katja Koštomaj, Tatjana Wulff, Tune Geirsdóttir, Margrét Almgren, Annette Jónsdóttir, Rósa Kristinsson, Hordur G. Undeland, Ingrid |
author_sort |
Jamnik, Polona |
title |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_short |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_full |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_fullStr |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_full_unstemmed |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_sort |
bioactivity of cod and chicken protein hydrolysates before and after in vitro gastrointestinal digestion |
publisher |
Faculty of Food Technology and Biotechnology, University of Zagreb |
publishDate |
2017 |
url |
https://hrcak.srce.hr/192536 https://hrcak.srce.hr/file/284070 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Food Technology and Biotechnology ISSN 1330-9862 (Print) ISSN 1334-2606 (Online) Volume 55 Issue 4 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.17113/ftb.55.03.17.5117 https://hrcak.srce.hr/192536 |
op_rights |
info:eu-repo/semantics/openAccess The contents of the Food Technology and Biotechnology can be used for personal, educational or scientific purposes, provided that the credit is given to the Journal (under Creative Commons licence CC BY-NC 4.0). Annual subscription price for all countries (except Croatia) is 40 euro for citizens and 100 euro for organizations and institutions. Discount is offered to the members of Croatian Society of Biotechnology and Slovenian Microbiological Society. For subscription please contact the Editorial Office at the E-mail address: ftb@pbf.hr |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.17113/ftb.55.03.17.5117 |
container_title |
Food Technology and Biotechnology |
container_volume |
55 |
container_issue |
3 |
_version_ |
1766005623048110080 |