Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion
Bioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and aft er in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellular expos...
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University of Zagreb
2017
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ftdoajarticles:oai:doaj.org/article:ebf5a1cf99554470b54d1fc0ecf85182 2023-05-15T16:19:18+02:00 Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion Polona Jamnik Katja Istenič Tatjana Koštomaj Tune Wulff Margrét Geirsdóttir Annette Almgren Rósa Jónsdóttir Hordur G. Kristinsson Ingrid Undeland 2017-01-01T00:00:00Z https://doaj.org/article/ebf5a1cf99554470b54d1fc0ecf85182 EN eng University of Zagreb http://hrcak.srce.hr/file/275142 https://doaj.org/toc/1330-9862 https://doaj.org/toc/1334-2606 1330-9862 1334-2606 https://doaj.org/article/ebf5a1cf99554470b54d1fc0ecf85182 Food Technology and Biotechnology, Vol 55, Iss 3, Pp 360-367 (2017) protein hydrolysates cod chicken in vitro gastrointestinal digestion yeast proteomics Biotechnology TP248.13-248.65 Food processing and manufacture TP368-456 article 2017 ftdoajarticles 2022-12-31T04:43:55Z Bioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and aft er in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellular exposure to simulate digestion conditions in the human body and therefore investigate the role of modulations in the GI tract on the cell response. The eff ect of digested and undigested hydrolysates on intracellular oxidation, cellular metabolic energy and proteome level was investigated. No diff erence in the eff ect on intracellular oxidation activity was obtained between cod and chicken hydrolysates, while higher aff ect on intracellular oxidation was provided by digested hydrolysates, with relative values of intracellular oxidation of cod of (70.2±0.8) and chicken of (74.5±1.4) % than by undigested ones, where values of cod and chicken were (95.5±1.2) and (90.5±0.7) %, respectively. Neither species nor digestion had any eff ect on cellular metabolic energy. At proteome level, digested hydrolysates gave again signifi cantly stronger responses than undigested counterparts; cod peptides here also gave somewhat stronger response than chicken peptides. The knowledge of the action of food protein hydrolysates and their digests within live cells, also at proteome level, is important for further validation of their activity in higher eukaryotes to develop new functional food ingredients, such as in this case chicken and cod muscle-derived peptides. Article in Journal/Newspaper Gadus morhua Directory of Open Access Journals: DOAJ Articles |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
protein hydrolysates cod chicken in vitro gastrointestinal digestion yeast proteomics Biotechnology TP248.13-248.65 Food processing and manufacture TP368-456 |
spellingShingle |
protein hydrolysates cod chicken in vitro gastrointestinal digestion yeast proteomics Biotechnology TP248.13-248.65 Food processing and manufacture TP368-456 Polona Jamnik Katja Istenič Tatjana Koštomaj Tune Wulff Margrét Geirsdóttir Annette Almgren Rósa Jónsdóttir Hordur G. Kristinsson Ingrid Undeland Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
topic_facet |
protein hydrolysates cod chicken in vitro gastrointestinal digestion yeast proteomics Biotechnology TP248.13-248.65 Food processing and manufacture TP368-456 |
description |
Bioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and aft er in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellular exposure to simulate digestion conditions in the human body and therefore investigate the role of modulations in the GI tract on the cell response. The eff ect of digested and undigested hydrolysates on intracellular oxidation, cellular metabolic energy and proteome level was investigated. No diff erence in the eff ect on intracellular oxidation activity was obtained between cod and chicken hydrolysates, while higher aff ect on intracellular oxidation was provided by digested hydrolysates, with relative values of intracellular oxidation of cod of (70.2±0.8) and chicken of (74.5±1.4) % than by undigested ones, where values of cod and chicken were (95.5±1.2) and (90.5±0.7) %, respectively. Neither species nor digestion had any eff ect on cellular metabolic energy. At proteome level, digested hydrolysates gave again signifi cantly stronger responses than undigested counterparts; cod peptides here also gave somewhat stronger response than chicken peptides. The knowledge of the action of food protein hydrolysates and their digests within live cells, also at proteome level, is important for further validation of their activity in higher eukaryotes to develop new functional food ingredients, such as in this case chicken and cod muscle-derived peptides. |
format |
Article in Journal/Newspaper |
author |
Polona Jamnik Katja Istenič Tatjana Koštomaj Tune Wulff Margrét Geirsdóttir Annette Almgren Rósa Jónsdóttir Hordur G. Kristinsson Ingrid Undeland |
author_facet |
Polona Jamnik Katja Istenič Tatjana Koštomaj Tune Wulff Margrét Geirsdóttir Annette Almgren Rósa Jónsdóttir Hordur G. Kristinsson Ingrid Undeland |
author_sort |
Polona Jamnik |
title |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_short |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_full |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_fullStr |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_full_unstemmed |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_sort |
bioactivity of cod and chicken protein hydrolysates before and after in vitro gastrointestinal digestion |
publisher |
University of Zagreb |
publishDate |
2017 |
url |
https://doaj.org/article/ebf5a1cf99554470b54d1fc0ecf85182 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Food Technology and Biotechnology, Vol 55, Iss 3, Pp 360-367 (2017) |
op_relation |
http://hrcak.srce.hr/file/275142 https://doaj.org/toc/1330-9862 https://doaj.org/toc/1334-2606 1330-9862 1334-2606 https://doaj.org/article/ebf5a1cf99554470b54d1fc0ecf85182 |
_version_ |
1766005679420604416 |