Selection and Optimization of Medium Components for the Efficient Production of L-Asparaginase by Leucosporidium scottii L115—A Psychrotolerant Yeast

• Published in: Fermentation
• Main Authors: Ignacio S. Moguel, Celina K. Yamakawa, Larissa P. Brumano, Adalberto Pessoa, Solange I. Mussatto
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2022
• Subjects: L-asparaginase; medium composition; optimization; Leucosporidium scottii; psychrotolerant; Fermentation industries. Beverages. Alcohol; TP500-660; Antarctic; The Antarctic; Antarc*
• Online Access: https://doi.org/10.3390/fermentation8080398; https://doaj.org/article/176c7f024f104ae3a1153ec7a0c82dc1

This study reports the production of L-asparaginase (ASNase), an enzyme mainly used for the treatment of acute lymphoblastic leukemia, by Leucosporidium scottii L115, a psychrotolerant yeast isolated from the Antarctic ecosystem. Focus was given to select the most appropriate medium components able to maximize the enzyme production by this yeast, as a first step for the development of a new process to produce ASNase. By combining knowledge in bioprocesses, statistical analysis and modeling, the medium composition that most favored enzyme production was established, which consisted of using a mixture of sucrose (28.34 g L −1 ) and glycerol (15.61 g L −1 ) as carbon sources, supplemented with proline (6.15 g L −1 ) and the following salts (g L −1 ): KCl, 0.52; MgSO 4 ·7H 2 O, 0.52; CuNO 3 ·3H 2 O, 0.001; ZnSO 4 ·7H 2 O, 0.001; and FeSO 4 ·7H 2 O, 0.001. By using this medium, enzyme production of 2850 U L −1 (productivity of 23.75 U L −1 h −1 ) was obtained, which represented a 28-fold increase in enzyme production per gram of cells (178 U gdcw −1 ) when compared to the control (non-optimized medium), and a 50-fold increase when compared to a reference medium used for ASNase production.