Tropomyosin, the Major Oyster Crassostrea gigas Allergen and its IgE‐binding Epitopes
ABSTRACT The major allergen ( Cra g 1) isolated from the oyster Crassostrea gigas was digested with lysylendopeptidase. Twenty‐one peptide fragments were isolated from the digest by reverse‐phase HPLC and some of them were sequenced. Cra g 1 was identified as tropomyosin. In competitive ELISA inhibi...
| Published in: | Journal of Food Science |
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| Main Authors: | , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
1998
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1111/j.1365-2621.1998.tb15672.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2621.1998.tb15672.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2621.1998.tb15672.x/fullpdf |
| Summary: | ABSTRACT The major allergen ( Cra g 1) isolated from the oyster Crassostrea gigas was digested with lysylendopeptidase. Twenty‐one peptide fragments were isolated from the digest by reverse‐phase HPLC and some of them were sequenced. Cra g 1 was identified as tropomyosin. In competitive ELISA inhibition analyses of lysylendopeptidase fragments, a peptide (K21) showed maximum inhibition of the binding of IgE antibodies in sensitive human serum to Cra g 1. On further tryptic hydrolysis of K21, two peptides exhibited high inhibitory ability equivalent to K21. These results suggested that Cra g 1 had an IgE‐binding epitope in the segment 92–105 (IQLLEEDMERSEER) which was dissimilar to those of the major allergen (tropomyosin) from the shrimp Penaeus indicus. |
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