Eicosapentaenoic acid‐enriched phospholipids suppressed lipid accumulation by specific inhibition of lipid droplet‐associated protein FSP27 in mice
Abstract BACKGROUND Sea cucumber is a rich source of eicosapentaenoic acid in the form of eicosapentaenoic acid‐enriched phospholipids (EPA‐PL). It is known to be efficacious in preventing obesity. However, few studies have focused on the role of EPA‐PL in inhibiting lipid accumulation by lipid drop...
| Published in: | Journal of the Science of Food and Agriculture |
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| Main Authors: | , , , , , , |
| Other Authors: | |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
2020
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1002/jsfa.10250 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.10250 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jsfa.10250 |
| Summary: | Abstract BACKGROUND Sea cucumber is a rich source of eicosapentaenoic acid in the form of eicosapentaenoic acid‐enriched phospholipids (EPA‐PL). It is known to be efficacious in preventing obesity. However, few studies have focused on the role of EPA‐PL in inhibiting lipid accumulation by lipid droplets (LDs). This study first investigated the effect of EPA‐PL from sea cucumber on the formation of LDs and the underlying mechanism in C57BL/6J mice. The mice were randomly divided into two groups and treated for 8 weeks or 3, 7, and 14 days with either (i) a high‐sucrose diet (model group), (ii) a high‐sucrose diet plus 2% EPA‐PL (EPA‐PL group). RESULTS Eight‐week EPA‐PL supplementation significantly reduced lipid accumulation and LD size in liver and white adipose tissue (WAT), which was accompanied by the decreased expression of LDs‐associated protein FSP27. A 3‐day EPA‐PL treatment suppressed the mRNA expression of Fsp27 . The mRNA level of Fsp27 reached its ‘normal level’ after withdrawing EPA‐PL for 7 days, suggesting that EPA‐PL might serve as a rapid regulator of FSP27. Furthermore, EPA‐PL increased the expression of lipolysis genes Hsl and Atgl accompanied by the regulation of Pparγ in WAT. CONCLUSIONS Dietary EPA‐PL from sea cucumber ( Cucumaria frondosa ) protected against lipid accumulation by regulating LDs‐associated protein FSP27, which might provide novel evidence for the anti‐obesity action of EPA‐PL. © 2020 Society of Chemical Industry |
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