GIEMSA BANDING IN MALE RAT (RATTUS RATTUS)

ABSTRACT G-banding analysis was carried out on specimens of house rats. The diploid chromosome number of Rattus rattus is 2n=38 and the karyotype formula is 16m + 4sm+ 16a + 1X+1Y. The bands produced were specific for each chromosome pair in this animal. The karyotype by G-banding was prepared for m...

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Bibliographic Details
Main Author: Ashok J. Patil
Format: Article in Journal/Newspaper
Language:English
Published: 2022
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Online Access:https://zenodo.org/record/7220499
https://doi.org/10.5281/zenodo.7220499
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Summary:ABSTRACT G-banding analysis was carried out on specimens of house rats. The diploid chromosome number of Rattus rattus is 2n=38 and the karyotype formula is 16m + 4sm+ 16a + 1X+1Y. The bands produced were specific for each chromosome pair in this animal. The karyotype by G-banding was prepared for male rat including 36 autosomes contains 16 metacentric, 4 sub-metacentric and 16 acrocentric chromosomes which were arranged in decreasing order of each group. The male contain 2 different sex chromosomes the X which is sub-metacentric and Y chromosome is acrocentric which have arranged separately. Key Words: G-banding, Karyotype, Rattus rattus, Chromosome, Trypsin REFERENCES Aggrighi and Hsu 1971. Localization of heterochromatin in human chromosomes. Cytogenetics 10(2): 81-6 Badenhorst, D., Herbreleau V., Chaval Y., Pages M., Robinson T.J., Rerkamnuaychoke W., Morand S., Hugot J.P., Dobigny G. 2009. New Karyotypes data for Asian rodents (Rodentia, Muridae) with the first report of B-chromosomes in the genes Mus. Journal of Zoology, 279, 44-56. Chiarelli, B. 1973. Genan Phaenen. 16, 3 (cited in chromosome techniques by A.K. Sharma and A. Sharma–3rd edition Butterworth and Co. (Publishers) Ltd. London. Comings, D. E. 1973. In chromosome identification, eds. T. Caspersson and L. Zech. New York, Academic Press. Ellerman, J. R. 1961. Fauna of India. Mammalia Rodentia 2nd edition, Baptist Mission Press, Calcutta; 3 (2). Matsui, S. I. and Sasaki, M. S. 1975. The mechanism of Giemsa banding of mammalian chromosomes, with special attention to the role of non-histone proteins. Japan J. Genetics. 50 (3): 189-04. Miller, O. J. 1973. In chromosome identification, Nobel Symposia. 23, New York Academic Press. Paradue, M. L. and Gall, J. G. 1970. Small localization of mouse satellite DNA. Science. 168, 1356-58. Pearson, P. L and Van Egmond-Cowan, A. M. H. 1976. In new techniques in Biophysics and Cell Biology (Eds. Pain R.H. and Smith B.J. 3, 213, New York: John Willey. Prater, S. H. 1971. Indian animals. Curator, Bombay Natural ...