Data from: Nitrogen deposition alters plant–fungal relationships: linking belowground dynamics to aboveground vegetation change
Nitrogen (N) deposition rates are increasing globally due to anthropogenic activities. Plant community responses to N are often attributed to altered competitive interactions between plants, but may also be a result of microbial responses to N, particularly root-associated fungi (RAF), which are kno...
Main Authors: | , , , , , |
---|---|
Format: | Other/Unknown Material |
Language: | unknown |
Published: |
Zenodo
2013
|
Subjects: | |
Online Access: | https://doi.org/10.5061/dryad.sv33f |
id |
ftzenodo:oai:zenodo.org:4942355 |
---|---|
record_format |
openpolar |
spelling |
ftzenodo:oai:zenodo.org:4942355 2024-09-15T18:39:53+00:00 Data from: Nitrogen deposition alters plant–fungal relationships: linking belowground dynamics to aboveground vegetation change Dean, Sarah L. Farrer, Emily C. Taylor, D. Lee Porras-Alfaro, Andrea Suding, Katharine N. Sinsabaugh, Robert L. 2013-09-23 https://doi.org/10.5061/dryad.sv33f unknown Zenodo https://doi.org/10.1111/mec.12541 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.sv33f oai:zenodo.org:4942355 info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode Deschampsia cespitosa root fungi nitrogen deposition Geum rossii 454 pyrosequencing ITS Helotiales info:eu-repo/semantics/other 2013 ftzenodo https://doi.org/10.5061/dryad.sv33f10.1111/mec.12541 2024-07-26T07:19:13Z Nitrogen (N) deposition rates are increasing globally due to anthropogenic activities. Plant community responses to N are often attributed to altered competitive interactions between plants, but may also be a result of microbial responses to N, particularly root-associated fungi (RAF), which are known to affect plant fitness. In response to N, Deschampsia cespitosa, a codominant plant in the alpine tundra at Niwot Ridge (CO), increases in abundance, while Geum rossii, its principal competitor, declines. Importantly, G. rossii declines with N even in the absence of its competitor. We examined whether contrasting host responses to N are associated with altered plant–fungal symbioses, and whether the effects of N are distinct from effects of altered plant competition on RAF, using 454 pyrosequencing. Host RAF communities were distinct (only 9.4% of OTUs overlapped). N increased RAF diversity in G. rossii, but decreased it in D. cespitosa. D. cespitosa RAF communities were more responsive to N than G. rossii RAF communities, perhaps indicating a flexible microbial community aids host adaptation to nutrient enrichment. Effects of removing D. cespitosa were distinct from effects of N on G. rossii RAF, and D. cespitosa presence reversed RAF diversity response to N. The most dominant G. rossii RAF order, Helotiales, was the most affected by N, declining from 83% to 60% of sequences, perhaps indicating a loss of mutualists under N enrichment. These results highlight the potential importance of belowground microbial dynamics in plant responses to N deposition. alpha diversity code R code that tests for relationships between alpha diversity measures and treatment. Inputs are collated alpha diversity files produced in QIIME. alpha.R NiwotDGF map Environmental mapping file, providing treatment, plot, DNA dilution, and sequencing run information for each sample. NiwotDGF_map3.csv NMDS code R code to build NMDS plot of bray-curtis distance matrix describing distances between communities from each sample. Also a permutation ... Other/Unknown Material Tundra Zenodo |
institution |
Open Polar |
collection |
Zenodo |
op_collection_id |
ftzenodo |
language |
unknown |
topic |
Deschampsia cespitosa root fungi nitrogen deposition Geum rossii 454 pyrosequencing ITS Helotiales |
spellingShingle |
Deschampsia cespitosa root fungi nitrogen deposition Geum rossii 454 pyrosequencing ITS Helotiales Dean, Sarah L. Farrer, Emily C. Taylor, D. Lee Porras-Alfaro, Andrea Suding, Katharine N. Sinsabaugh, Robert L. Data from: Nitrogen deposition alters plant–fungal relationships: linking belowground dynamics to aboveground vegetation change |
topic_facet |
Deschampsia cespitosa root fungi nitrogen deposition Geum rossii 454 pyrosequencing ITS Helotiales |
description |
Nitrogen (N) deposition rates are increasing globally due to anthropogenic activities. Plant community responses to N are often attributed to altered competitive interactions between plants, but may also be a result of microbial responses to N, particularly root-associated fungi (RAF), which are known to affect plant fitness. In response to N, Deschampsia cespitosa, a codominant plant in the alpine tundra at Niwot Ridge (CO), increases in abundance, while Geum rossii, its principal competitor, declines. Importantly, G. rossii declines with N even in the absence of its competitor. We examined whether contrasting host responses to N are associated with altered plant–fungal symbioses, and whether the effects of N are distinct from effects of altered plant competition on RAF, using 454 pyrosequencing. Host RAF communities were distinct (only 9.4% of OTUs overlapped). N increased RAF diversity in G. rossii, but decreased it in D. cespitosa. D. cespitosa RAF communities were more responsive to N than G. rossii RAF communities, perhaps indicating a flexible microbial community aids host adaptation to nutrient enrichment. Effects of removing D. cespitosa were distinct from effects of N on G. rossii RAF, and D. cespitosa presence reversed RAF diversity response to N. The most dominant G. rossii RAF order, Helotiales, was the most affected by N, declining from 83% to 60% of sequences, perhaps indicating a loss of mutualists under N enrichment. These results highlight the potential importance of belowground microbial dynamics in plant responses to N deposition. alpha diversity code R code that tests for relationships between alpha diversity measures and treatment. Inputs are collated alpha diversity files produced in QIIME. alpha.R NiwotDGF map Environmental mapping file, providing treatment, plot, DNA dilution, and sequencing run information for each sample. NiwotDGF_map3.csv NMDS code R code to build NMDS plot of bray-curtis distance matrix describing distances between communities from each sample. Also a permutation ... |
format |
Other/Unknown Material |
author |
Dean, Sarah L. Farrer, Emily C. Taylor, D. Lee Porras-Alfaro, Andrea Suding, Katharine N. Sinsabaugh, Robert L. |
author_facet |
Dean, Sarah L. Farrer, Emily C. Taylor, D. Lee Porras-Alfaro, Andrea Suding, Katharine N. Sinsabaugh, Robert L. |
author_sort |
Dean, Sarah L. |
title |
Data from: Nitrogen deposition alters plant–fungal relationships: linking belowground dynamics to aboveground vegetation change |
title_short |
Data from: Nitrogen deposition alters plant–fungal relationships: linking belowground dynamics to aboveground vegetation change |
title_full |
Data from: Nitrogen deposition alters plant–fungal relationships: linking belowground dynamics to aboveground vegetation change |
title_fullStr |
Data from: Nitrogen deposition alters plant–fungal relationships: linking belowground dynamics to aboveground vegetation change |
title_full_unstemmed |
Data from: Nitrogen deposition alters plant–fungal relationships: linking belowground dynamics to aboveground vegetation change |
title_sort |
data from: nitrogen deposition alters plant–fungal relationships: linking belowground dynamics to aboveground vegetation change |
publisher |
Zenodo |
publishDate |
2013 |
url |
https://doi.org/10.5061/dryad.sv33f |
genre |
Tundra |
genre_facet |
Tundra |
op_relation |
https://doi.org/10.1111/mec.12541 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.sv33f oai:zenodo.org:4942355 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.sv33f10.1111/mec.12541 |
_version_ |
1810484232090288128 |