Dactylogyrus skrjabini Achmerow 1954
Dactylogyrus skrjabini Achmerow, 1954 [New Japanese name: Dai-yubigata-mushi] (Fig. 2) Dactylogyrus skrjabini Achmerow, 1954: 167–168, fig. 1; Bogdanova 1957: 1391–1393; Long and Lee 1960: 218–219, fig. 2; Akmetov 1963: 462; Lee 1963: 76; Yamaguti 1963a: 30; Musselius 1969: 237–238, 240; Bauer and H...
| Main Authors: | , |
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| Format: | Other/Unknown Material |
| Language: | unknown |
| Published: |
2020
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| Subjects: | |
| Online Access: | https://zenodo.org/record/4734633 https://doi.org/10.5281/zenodo.4734633 |
| Summary: | Dactylogyrus skrjabini Achmerow, 1954 [New Japanese name: Dai-yubigata-mushi] (Fig. 2) Dactylogyrus skrjabini Achmerow, 1954: 167–168, fig. 1; Bogdanova 1957: 1391–1393; Long and Lee 1960: 218–219, fig. 2; Akmetov 1963: 462; Lee 1963: 76; Yamaguti 1963a: 30; Musselius 1969: 237–238, 240; Bauer and Hoffman 1976: 165; Gvozdev and Agapova 1977: 109; Rohde 1979: 655; Hoffman and Schubert 1984: 238; Ali et al. 1989: 152–153; Gibson et al. 1996: 29; Blanc 1997: 497; Xia et al. 2000: 152; Grigorovich et al. 2002: 1208; Johnson and Lunde 2005: 132; Al-Saadi et al. 2010: 3, 4; Karabekova 2008: 331, 333; Mhaisen et al. 2012: 107, 116; Zhang 2012: 123; Al-Jawda and Asmar 2015: 129; Mhaisen and Al-Rubaie 2016: 5, 7. Copepods were removed from the gills using small needles and forceps and fixed in 70 or 99% ethanol. Copepods were cleared and dissected in lactic acid. The whole body was examined using the wooden slide method (Humes and Gooding 1964). The removed appendages and parts of the body were dehydrated through a graded ethanol series, cleared in xylene, mounted in Canada balsam, and examined for morphological characters. Drawings were made with the aid of a drawing tube fitted on an Olympus BX51 light microscope. Measurements, in micrometers, are expressed as the range. The monogenean and copepod specimens are deposited in the Platyhelminthes and Crustacea collections of the National Museum of Nature and Science (NSMT-Pl and NSMT-Cr), Tsukuba City, Ibaraki Prefecture, Japan, respectively. DNA was extracted from two specimens of D. skrjabini using the DNeasy blood and tissue kit (Qiagen) in accordance with the manufacturer’s instructions. The DNA was amplified by polymerase chain reaction (PCR) using the primer pair C1 (5′ -ACC CGC TGA ATT TAA GCA T- 3′) and D2 (5′ -TGG TCC GTG TTT CAA GAC- 3′) to amplify partial 28S rDNA (Vân Le et al. 1993). A total of 25 µL PCR reaction consisted of 1 µL of DNA template, 10×Titanium Taq PCR Buffer (Clonetech), 0.2 mM of each dNTP, 1 μ M of each primer, and 1×Titanium Taq DNA ... |
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