LC-ESI-MS/MS analysis of GlcNAc-Transferase I activity

The dabsylated acceptor Man5GlcNAc2-Asn was incubated with recombinat UDP-N-acetylglucosamine:α-1,3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I from the Pacific oyster, Crassostrea gigas in order to test the enzyme´s substrat specificity. The substrate and the product were analysed by LC-ESI...

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Main Author: Core Facility Mass Spectrometry, BOKU
Other Authors: Thoma, Julia, Staudacher, Erika
Format: Other/Unknown Material
Language:English
Published: Zenodo 2023
Subjects:
Online Access:https://doi.org/10.5281/zenodo.13748199
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author Core Facility Mass Spectrometry, BOKU
author2 Thoma, Julia
Staudacher, Erika
author_facet Core Facility Mass Spectrometry, BOKU
author_sort Core Facility Mass Spectrometry, BOKU
collection Zenodo
description The dabsylated acceptor Man5GlcNAc2-Asn was incubated with recombinat UDP-N-acetylglucosamine:α-1,3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I from the Pacific oyster, Crassostrea gigas in order to test the enzyme´s substrat specificity. The substrate and the product were analysed by LC-ESI-MS/MS.
format Other/Unknown Material
genre Crassostrea gigas
Pacific oyster
genre_facet Crassostrea gigas
Pacific oyster
geographic Pacific
geographic_facet Pacific
id ftzenodo:oai:zenodo.org:13748199
institution Open Polar
language English
op_collection_id ftzenodo
op_doi https://doi.org/10.5281/zenodo.1374819910.1007/s10719-024-10148-910.5281/zenodo.13748198
op_relation https://doi.org/10.1007/s10719-024-10148-9
https://doi.org/10.5281/zenodo.13748198
https://doi.org/10.5281/zenodo.13748199
oai:zenodo.org:13748199
op_rights info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
op_source Glycoconj. J., 41(2), 151-162, (2023-01-31)
publishDate 2023
publisher Zenodo
record_format openpolar
spelling ftzenodo:oai:zenodo.org:13748199 2025-01-16T21:33:28+00:00 LC-ESI-MS/MS analysis of GlcNAc-Transferase I activity Core Facility Mass Spectrometry, BOKU Thoma, Julia Staudacher, Erika 2023-01-31 https://doi.org/10.5281/zenodo.13748199 eng eng Zenodo https://doi.org/10.1007/s10719-024-10148-9 https://doi.org/10.5281/zenodo.13748198 https://doi.org/10.5281/zenodo.13748199 oai:zenodo.org:13748199 info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode Glycoconj. J., 41(2), 151-162, (2023-01-31) Crassostrea gigas Glycosyltransferase Mollusca UDP-N-acetylglucosamine:α-1,3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I info:eu-repo/semantics/other 2023 ftzenodo https://doi.org/10.5281/zenodo.1374819910.1007/s10719-024-10148-910.5281/zenodo.13748198 2024-12-05T14:59:50Z The dabsylated acceptor Man5GlcNAc2-Asn was incubated with recombinat UDP-N-acetylglucosamine:α-1,3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I from the Pacific oyster, Crassostrea gigas in order to test the enzyme´s substrat specificity. The substrate and the product were analysed by LC-ESI-MS/MS. Other/Unknown Material Crassostrea gigas Pacific oyster Zenodo Pacific
spellingShingle Crassostrea gigas
Glycosyltransferase
Mollusca
UDP-N-acetylglucosamine:α-1,3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I
Core Facility Mass Spectrometry, BOKU
LC-ESI-MS/MS analysis of GlcNAc-Transferase I activity
title LC-ESI-MS/MS analysis of GlcNAc-Transferase I activity
title_full LC-ESI-MS/MS analysis of GlcNAc-Transferase I activity
title_fullStr LC-ESI-MS/MS analysis of GlcNAc-Transferase I activity
title_full_unstemmed LC-ESI-MS/MS analysis of GlcNAc-Transferase I activity
title_short LC-ESI-MS/MS analysis of GlcNAc-Transferase I activity
title_sort lc-esi-ms/ms analysis of glcnac-transferase i activity
topic Crassostrea gigas
Glycosyltransferase
Mollusca
UDP-N-acetylglucosamine:α-1,3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I
topic_facet Crassostrea gigas
Glycosyltransferase
Mollusca
UDP-N-acetylglucosamine:α-1,3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I
url https://doi.org/10.5281/zenodo.13748199