LC-ESI-MS/MS analysis of GlcNAc-Transferase I activity

The dabsylated acceptor Man5GlcNAc2-Asn was incubated with recombinat UDP-N-acetylglucosamine:α-1,3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I from the Pacific oyster, Crassostrea gigas in order to test the enzyme´s substrat specificity. The substrate and the product were analysed by LC-ESI...

Full description

Bibliographic Details
Main Author: Core Facility Mass Spectrometry, BOKU
Other Authors: Thoma, Julia, Staudacher, Erika
Format: Other/Unknown Material
Language:English
Published: Zenodo 2023
Subjects:
Crassostrea gigas
Glycosyltransferase
Mollusca
UDP-N-acetylglucosamine:α-1,3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I
Pacific
Pacific oyster
Online Access:https://doi.org/10.5281/zenodo.13748199
Description
Summary:The dabsylated acceptor Man5GlcNAc2-Asn was incubated with recombinat UDP-N-acetylglucosamine:α-1,3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I from the Pacific oyster, Crassostrea gigas in order to test the enzyme´s substrat specificity. The substrate and the product were analysed by LC-ESI-MS/MS.

Similar Items