| Summary: | This presentation was delivered at the ASP 99th Annual Meeting (Denver, Colorado, USA) by Dr. Philippe Vieira Alves (Dept. of Parasitology, São Paulo State University-UNESP). AUTHORS Philippe V. Alves(1,2), Reinaldo J. da Silva(1), Daniel Janies (2,3), Willian Taylor(3), April Harris(3), Gari New(3), Denis J. Machado (2,3). INSTITUTIONS (1) São Paulo State University (UNESP), Institute of Biosciences, Section of Parasitology, Botucatu, SP, Brazil; (2) University of North Carolina at Charlotte, Center for Computational Intelligence to Predict Health and Environmental Risks (CIPHER), Charlotte, NC, USA (3) University of North Carolina at Charlotte (UNC Charlotte), Department of Bioinformatics & Genomics (BiG) TITLE Mitogenome organization, diversity, and evolutionary relationships of proteocephalidean tapeworms (Cestoda, Onchoproteocephalidea) unveiled by a genome skimming approach ABSTRACT Proteocephalidean tapeworms (Onchoproteocephalidea I) are a diverse group of acetabulate cestodes that primarily infect bony fishes, snakes, and lizards worldwide. While molecular data, mainly from Sanger sequencing of the nuclear 28S rRNA and the mitochondrial MT-CO1, has been crucial to a better understanding of the taxonomy and systematics of proteocephalids, long-standing questions on the evolution of this taxon remain to be answered. Here, we subjected ethanol-preserved proteocephalid specimens to high-throughput DNA sequencing, using a genome skimming approach, to unravel the mitogenome organization and diversity of dozens of species. Posteriormost proglottids of vouchered specimens were provided by the Natural History Museum (Platyhelminthes collection), Geneva, Switzerland (MHNG-PLAT), and the Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic (IPCAS). Of 96 Illumina sequenced libraries, 90 yielded complete or partial mitogenomes. These 90 mitogenomes correspond to 83 species in 40 genera from all continents except Antarctica, including parasites of fishes ...
|
|---|