Setaria tundra
3. Results and discussion The morphology of worms isolated from the peritoneal cavity was identical to S. tundra by light microscopy (Fig. 2). This was confirmed by sequences of the 12S rRNA and cox1 genes, which were 99.1 ‾ 99.8% identical to previously published S. tundra isolates from Germany, Fr...
| Main Authors: | , , , , , |
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| Format: | Other/Unknown Material |
| Language: | unknown |
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Zenodo
2017
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| Subjects: | |
| Online Access: | https://doi.org/10.5281/zenodo.10966663 http://treatment.plazi.org/id/03D8879C3171FFD9FCE1F9D5FC20FA4E |
| Summary: | 3. Results and discussion The morphology of worms isolated from the peritoneal cavity was identical to S. tundra by light microscopy (Fig. 2). This was confirmed by sequences of the 12S rRNA and cox1 genes, which were 99.1 ‾ 99.8% identical to previously published S. tundra isolates from Germany, France, Italy, Spain and Finland. Phylogenetic trees constructed based on 12S rRNA and cox1 sequences were similar in topology and therefore only one tree is presented (Fig. 3). Accordingly, the Danish isolates described here were clearly grouped with other isolates of S. tundra from Europe in one node that had high bootstrap values for NJ (0.99). However, sequence analysis showed slight variability within the Danish isolates, which was reflected in their topology in the phylogenetic tree. For example, the Danish isolates 2013-410-1 & 2, which geographically originated from Zealand (an island) were more closely related to the isolate 2010- 1233 that originated from Jutland (a peninsula) than the other isolate (2012-1665) from Zealand. The Danish isolate 2012-1665, with worms encapsulated in the liver capsule, had 0.5% variation in the cox1 compared to other Danish isolates, but had a lower level of variability (0.2 ‾ 0.3%) and was phylogenetically more closely related to other European isolates (Fig. 3). Despite the low bootstrap values, the general topology of the Setaria spp. resembles a previously published tree by Alasaad et al. (2012). This is the first report of S. tundra in Denmark. Worms of Setaria transcaucasica were earlier recovered from 41 out of 76 roe deer (53.9%) collected from the island LaesØ in Denmark (Korsholm, 1988). In that study, the worms recovered from younger animals were encapsulated in different visceral organs and tissues, whereas in older animals the worms were found free in the peritoneal cavity. It is hard to determine if the proposed identification of the parasites was valid in the study by Korsholm (1988) because of the similar position of the worms in the subcapsular layer of ... |
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