| Summary: | Wound healing is a complex and dynamic process of replacing devitalized and missing cellular structures and tissue layers. We have previously shown that melanin, producer of which is the Antarctic black yeast fungi Pseudonadsoniella brunea (Nadsoniella nigra sp. X-1), sown with samples of vertical cliffs of the island Galindez (Ukrainian Antarctic Station “Akademik Vernadsky”) has expressed cytoprotective effect, promoted rapid wound healing of various ethiology and can be offered as a new dermatropic drug. We have created a new pharmacological composition which includes 0,1% melanin, dissolved in 0,5% Carbopol. Experiments conducted in accordance with international principles of the European Convention for the Protection of vertebrate animals used for experimental and other scientific purposes, according to the Law of Ukraine of 21.02.2006 № 3447-IV “On protection of animals from cruelty”. Research was conducted on white laboratory female rats weighing 200-250 g, which were divided into four groups. In each model animals experimental skin wounds without drugs were used as a control (first group). Wounds of rats of second group were treated only with 0,5% carbopol (universal solvent drugs to make them gel-like consistency, Carbopol 980”). Animals of third group got 0,1% melanin (produced by Antarctic black yeast-like fungi Nadsoniella nigra, strain X1-M,and received by us microbiologically) dissolved in 0,5% carbopol for wounds’ healing. Animals of fourth group without experimental skin wounds were used as a intact animals. Before the experiment, the rats were kept in quarantine and were marked by given them notches on ears. When animas were injured they were anesthetized by sodium thiopental (BiochemieGmbH/Austria), at a dosage of 50 mg/kg. Before the experiment epilation was performed in the shoulder-blade area. Model of full-thickness skin wound. Plate wounds are reproduced on epilated skin in anesthetized rats. To do this, skin is cut using surgical scalpel and forceps, 1 × 1sm2. Chemical skin burns were ...
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