Kinetics study of vitamin A precursor synthesis by immobilized lipasecatalyzed regioselective monoacetylation in n-hexane
Conference Name:4th International Conference on Manufacturing Science and Engineering, ICMSE 2013. Conference Address: Dalian, China. Time:March 30, 2013 - March 31, 2013. Northeastern University, China; Harbin Institute of Technology; Jilin University Vitamin A is an essential nutrient element in a...
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ftxiamenuniv:oai:dspace.xmu.edu.cn:2288/85407 2023-05-15T13:51:20+02:00 Kinetics study of vitamin A precursor synthesis by immobilized lipasecatalyzed regioselective monoacetylation in n-hexane Jing, Ke-Ju Duan, Ran Sun, Jin-Peng Wang, Shi-Zhen Lu, Ying-Hua 卢英华 2013 http://dspace.xmu.edu.cn/handle/2288/85407 en_US eng Trans Tech Publications Ltd Advanced Materials Research, 2013,690 693:1218-1223 1022-6680 20132416414895 http://dspace.xmu.edu.cn/handle/2288/85407 http://dx.doi.org/10.4028/www.scientific.net/AMR.690-693.1218 Acetylation Biosynthesis Bismuth Catalysis Hexane Isomers Kinetics Conference 2013 ftxiamenuniv 2020-07-21T11:40:42Z Conference Name:4th International Conference on Manufacturing Science and Engineering, ICMSE 2013. Conference Address: Dalian, China. Time:March 30, 2013 - March 31, 2013. Northeastern University, China; Harbin Institute of Technology; Jilin University Vitamin A is an essential nutrient element in animal and human growth, which is usually produced by partially acetylating and transforming retinyl diol. The lipase-catalyzed monoacetylation can obtain pure monoacetate compared with the classical chemistry process. In the current work, the synthesis of vitamin A precursor of Candida antarctica lipase B catalyzed by regioselective monoacetylation of primary hydroxyl of diol in n-hexane was studied. The reaction rate could be described in terms of the Michaelis-Menten equation with a Ping-Pong Bi-Bi mechanism and competitive inhibition by both substrates. A kinetic model was developed, and the apparent kinetic parameters were calculated as: Vmax =8.45 mmol/ (L h); Km,vinyl =0.997 mmol/L; Km,diol =161.28 mmol/L; Ki,diol =287.32 mmol/L; Ki,monoacetate=18.13 mmol/L; and KI,diol =427.40 mmol/L. The current study indicates a competitive enzyme inhibition of highly concentrated diol during lipase-catalyzed acetylation reaction. When the diol concentration in the medium was low, there was a good conformity between the experimental and simulated values with 4.73% average relative error. ? (2013) Trans Tech Publications, Switzerland. Conference Object Antarc* Antarctica Xiamen University Institutional Repository |
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Xiamen University Institutional Repository |
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ftxiamenuniv |
language |
English |
topic |
Acetylation Biosynthesis Bismuth Catalysis Hexane Isomers Kinetics |
spellingShingle |
Acetylation Biosynthesis Bismuth Catalysis Hexane Isomers Kinetics Jing, Ke-Ju Duan, Ran Sun, Jin-Peng Wang, Shi-Zhen Lu, Ying-Hua 卢英华 Kinetics study of vitamin A precursor synthesis by immobilized lipasecatalyzed regioselective monoacetylation in n-hexane |
topic_facet |
Acetylation Biosynthesis Bismuth Catalysis Hexane Isomers Kinetics |
description |
Conference Name:4th International Conference on Manufacturing Science and Engineering, ICMSE 2013. Conference Address: Dalian, China. Time:March 30, 2013 - March 31, 2013. Northeastern University, China; Harbin Institute of Technology; Jilin University Vitamin A is an essential nutrient element in animal and human growth, which is usually produced by partially acetylating and transforming retinyl diol. The lipase-catalyzed monoacetylation can obtain pure monoacetate compared with the classical chemistry process. In the current work, the synthesis of vitamin A precursor of Candida antarctica lipase B catalyzed by regioselective monoacetylation of primary hydroxyl of diol in n-hexane was studied. The reaction rate could be described in terms of the Michaelis-Menten equation with a Ping-Pong Bi-Bi mechanism and competitive inhibition by both substrates. A kinetic model was developed, and the apparent kinetic parameters were calculated as: Vmax =8.45 mmol/ (L h); Km,vinyl =0.997 mmol/L; Km,diol =161.28 mmol/L; Ki,diol =287.32 mmol/L; Ki,monoacetate=18.13 mmol/L; and KI,diol =427.40 mmol/L. The current study indicates a competitive enzyme inhibition of highly concentrated diol during lipase-catalyzed acetylation reaction. When the diol concentration in the medium was low, there was a good conformity between the experimental and simulated values with 4.73% average relative error. ? (2013) Trans Tech Publications, Switzerland. |
format |
Conference Object |
author |
Jing, Ke-Ju Duan, Ran Sun, Jin-Peng Wang, Shi-Zhen Lu, Ying-Hua 卢英华 |
author_facet |
Jing, Ke-Ju Duan, Ran Sun, Jin-Peng Wang, Shi-Zhen Lu, Ying-Hua 卢英华 |
author_sort |
Jing, Ke-Ju |
title |
Kinetics study of vitamin A precursor synthesis by immobilized lipasecatalyzed regioselective monoacetylation in n-hexane |
title_short |
Kinetics study of vitamin A precursor synthesis by immobilized lipasecatalyzed regioselective monoacetylation in n-hexane |
title_full |
Kinetics study of vitamin A precursor synthesis by immobilized lipasecatalyzed regioselective monoacetylation in n-hexane |
title_fullStr |
Kinetics study of vitamin A precursor synthesis by immobilized lipasecatalyzed regioselective monoacetylation in n-hexane |
title_full_unstemmed |
Kinetics study of vitamin A precursor synthesis by immobilized lipasecatalyzed regioselective monoacetylation in n-hexane |
title_sort |
kinetics study of vitamin a precursor synthesis by immobilized lipasecatalyzed regioselective monoacetylation in n-hexane |
publisher |
Trans Tech Publications Ltd |
publishDate |
2013 |
url |
http://dspace.xmu.edu.cn/handle/2288/85407 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
http://dx.doi.org/10.4028/www.scientific.net/AMR.690-693.1218 |
op_relation |
Advanced Materials Research, 2013,690 693:1218-1223 1022-6680 20132416414895 http://dspace.xmu.edu.cn/handle/2288/85407 |
_version_ |
1766255147216797696 |