| Summary: | Conference Name:4th International Conference on Manufacturing Science and Engineering, ICMSE 2013. Conference Address: Dalian, China. Time:March 30, 2013 - March 31, 2013. Northeastern University, China; Harbin Institute of Technology; Jilin University Vitamin A is an essential nutrient element in animal and human growth, which is usually produced by partially acetylating and transforming retinyl diol. The lipase-catalyzed monoacetylation can obtain pure monoacetate compared with the classical chemistry process. In the current work, the synthesis of vitamin A precursor of Candida antarctica lipase B catalyzed by regioselective monoacetylation of primary hydroxyl of diol in n-hexane was studied. The reaction rate could be described in terms of the Michaelis-Menten equation with a Ping-Pong Bi-Bi mechanism and competitive inhibition by both substrates. A kinetic model was developed, and the apparent kinetic parameters were calculated as: Vmax =8.45 mmol/ (L h); Km,vinyl =0.997 mmol/L; Km,diol =161.28 mmol/L; Ki,diol =287.32 mmol/L; Ki,monoacetate=18.13 mmol/L; and KI,diol =427.40 mmol/L. The current study indicates a competitive enzyme inhibition of highly concentrated diol during lipase-catalyzed acetylation reaction. When the diol concentration in the medium was low, there was a good conformity between the experimental and simulated values with 4.73% average relative error. ? (2013) Trans Tech Publications, Switzerland.
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