Induction of gynogenetic diploids in the small abalone, Haliotis diversicolor supertexta

The conditions for induction of gynogentic haploids and restoration of gynogenetic diploids, were studied in the small abalone, Haliotis diversicolor supertexta. Gynogentic diploids were produced by first using chromosome inactivated sperm to fertilize normal eggs and then suppressing the second mei...

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Bibliographic Details
Main Authors: Cai, MY, Ke, CH, Wang, ZY, Zhou, SQ, Zhang, ZX, Wang, YL, Zhang, ZP, 张中新
Format: Article in Journal/Newspaper
Language:English
Published: 2004
Subjects:
PACIFIC ABALONE
ARTIFICIAL GYNOGENESIS
CRASSOSTREA-GIGAS
IRRADIATED SPERM
CYTOCHALASIN-B
MANIPULATION
OYSTER
PLOIDY
Pacific
Crassostrea gigas
Online Access:https://dspace.xmu.edu.cn/handle/2288/66181
Description
Summary:The conditions for induction of gynogentic haploids and restoration of gynogenetic diploids, were studied in the small abalone, Haliotis diversicolor supertexta. Gynogentic diploids were produced by first using chromosome inactivated sperm to fertilize normal eggs and then suppressing the second meiotic division of these eggs. Sperm chromosomes were effectively inactivated with UV irradiation for 50-90 sec at an intensity of 1075 mu W cm(-2.)s(-1). The decreased capacity of UV irradiated sperm to fertilize eggs could be improved to an acceptable level by increasing insemination concentration of sperm. Eggs fertilized with genetically inactivated sperm developed into abnormal larvae in the haploid state. The diploids were restored when the gynogenetically activated eggs were treated with 0.5 mg l(-1) cytochalasin B (CB) for 10 min from 7 min to 17 min post insemination.

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