白令海北部浮游细菌及表层沉积物细菌的系统发育多样性分析

1.通过PCR-DGGE分析,发现白令海北部底、表层海水中的细菌生物多样性水平总体较高,而且底层水体中细菌多样性指数及丰度明显高于表层水体;St.Lawrence岛北部7个站位的表层和底层水样,其丰度和多样性指数平均值低于南部区域;采样深度大于100m的3个站位的底层水样,其多样性指数随着深度的增加出现递增现象。获得的36条DGGE条带,其序列除了有2条属于环境样品中的未知菌群以外,涵盖了以Proteobacteria和Actinobacteria为主的6个门。其中,gamma-Proteobacteria和alpha-Proteobacterium分别以25.0%和22.2%占据优势地位。另...

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Bibliographic Details
Main Author: 邹扬
Other Authors: 郑天凌, 曾胤新
Format: Thesis
Language:Chinese
Published: 2009
Subjects:
Online Access:http://dspace.xmu.edu.cn/handle/2288/47120
Description
Summary:1.通过PCR-DGGE分析,发现白令海北部底、表层海水中的细菌生物多样性水平总体较高,而且底层水体中细菌多样性指数及丰度明显高于表层水体;St.Lawrence岛北部7个站位的表层和底层水样,其丰度和多样性指数平均值低于南部区域;采样深度大于100m的3个站位的底层水样,其多样性指数随着深度的增加出现递增现象。获得的36条DGGE条带,其序列除了有2条属于环境样品中的未知菌群以外,涵盖了以Proteobacteria和Actinobacteria为主的6个门。其中,gamma-Proteobacteria和alpha-Proteobacterium分别以25.0%和22.2%占据优势地位。另. Biodiversity of bacteria in the bottom and surface water, as well as the bacteria in the top layer of sediments from the Northern Bering Sea, were studied by a combination of PCR-DGGE, RFLP and 16S rDNA analysis. Shannon-wiener’s Index and software BandScan were used to evaluate the bacterial biodiversity. The main results were as follows: 1. DGGE analysis showed that the bacterial biodive. 学位:理学硕士 院系专业:生命科学学院生物学系_微生物学 学号:21720061152162