南极假丝酵母脂肪酶B的固定化及其应用研究

• Main Author: 林旺锦
• Other Authors: 卢英华
• Format: Thesis
• Language: Chinese
• Published: 2018
• Subjects: 固定化酶; 维生素A棕榈酸酯; 转酯化; 动力学; 反应器; 维生素A氢化物; 单酰化; Immobilized enzyme; Vitamin A palmitate; Transesterification; Kinetics; Reactor; Vitamin A hydride; Mono acetylation; Antarc*; Antarctica
• Online Access: https://dspace.xmu.edu.cn/handle/2288/168457

摘要 南极假丝酵母脂肪酶B（CandidaantarcticalipaseB，CALB）是工业上广泛应用的脂肪酶。维生素A棕榈酸酯是具有良好市场前景的维生素A衍生物。利用南极假丝酵母脂肪酶B催化合成维生素A棕榈酸酯具有条件温和，反应选择性高，副反应少，产品的稳定性好，绿色环保等优点。 本文首先培养重组毕赤酵母获得高浓度CALB的发酵上清液。发酵144h后，游离酶酶活可达108U/mL，目标蛋白占总蛋白含量的百分比为63.4%。通过对游离酶固定化方法的比较，发现与共价法相比，吸附法操作简单，同时对酶活破坏小。采用大孔吸附树脂HPD826吸附固定脂肪酶，其催化月桂酸酯化的活性可达9200PLU. Abstract Candida antarctica lipase B (CALB) has been widely used in industry and vitamin A palmitate has great market prospects. The synthesis of vitamin A palmitate catalyzed by Candida antarctica lipase B has many advantages including mild conditions, high reaction selectivity, less side effects, good product stability, environmental friendly etc. Firstly, the high concentration of CALB were o. 学位：工学硕士 院系专业：化学化工学院_化学工程 学号：20620131151487