| Summary: | 摘要 南极假丝酵母脂肪酶B（CandidaantarcticalipaseB，CALB）是工业上广泛应用的脂肪酶。维生素A棕榈酸酯是具有良好市场前景的维生素A衍生物。利用南极假丝酵母脂肪酶B催化合成维生素A棕榈酸酯具有条件温和，反应选择性高，副反应少，产品的稳定性好，绿色环保等优点。 本文首先培养重组毕赤酵母获得高浓度CALB的发酵上清液。发酵144h后，游离酶酶活可达108U/mL，目标蛋白占总蛋白含量的百分比为63.4%。通过对游离酶固定化方法的比较，发现与共价法相比，吸附法操作简单，同时对酶活破坏小。采用大孔吸附树脂HPD826吸附固定脂肪酶，其催化月桂酸酯化的活性可达9200PLU. Abstract Candida antarctica lipase B (CALB) has been widely used in industry and vitamin A palmitate has great market prospects. The synthesis of vitamin A palmitate catalyzed by Candida antarctica lipase B has many advantages including mild conditions, high reaction selectivity, less side effects, good product stability, environmental friendly etc. Firstly, the high concentration of CALB were o. 学位：工学硕士 院系专业：化学化工学院_化学工程 学号：20620131151487
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