Sequencing and de novo Analysis of Crassostrea angulata (Fujian Oyster) from 8 Different Developing Phases Using 454 GSFlx

Research on the mechanism for early development of shellfish, such as body plan, shell formation, settlement and metamorphosis is currently an active research field. However, studies were still limited and not deep enough because of the lack of genomic resources such as genome or transcriptome seque...

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Bibliographic Details
Published in:PLoS ONE
Main Authors: Qin, Ji, Huang, Zixia, Chen, Jun, Zou, Quan, You, Weiwei, 游伟伟, Ke, Caihuan, 柯才焕
Format: Article in Journal/Newspaper
Language:English
Published: PUBLIC LIBRARY SCIENCE 2012
Subjects:
RECEPTOR-RELATED RECEPTOR
INSULIN-LIKE SYSTEM
PACIFIC OYSTER
HALIOTIS-TUBERCULATA
GASTROPOD MOLLUSK
GIGAS
TRANSCRIPTOME
EXPRESSION
METAMORPHOSIS
MANTLE
Pacific
Pacific oyster
Online Access:https://doi.org/10.1371/journal.pone.0043653
http://dspace.xmu.edu.cn/handle/2288/14892
Description
Summary:Research on the mechanism for early development of shellfish, such as body plan, shell formation, settlement and metamorphosis is currently an active research field. However, studies were still limited and not deep enough because of the lack of genomic resources such as genome or transcriptome sequences. In the present research, de novo transcriptome sequencing was performed for Crassostrea angulata, the most economically important cultured oyster species in China, at eight early developmental stages using the 454 sequencing technology. A total of 555,215 reads were produced with an average length of 309 nucleotides that were then assembled into 10,462 contigs. As determined by GO annotation and KEGG pathway mapping, functional annotation of the unigenes recovered diverse biological functions and processes. Six unique sequences related to settlement, metamorphosis and growth were subsequently analyzed by real-time PCR. Given the lack of whole genome information for oysters, transcriptome and de novo analysis of C. angulata from the eight different developing phases will provide important and useful information on early development mechanism and help genetic breeding of shellfish. National Basic Research Program of China [2010CB1264000]; Hi-Tech Research and Development (863) Program of China [2010AA10A110]; Shellfish Modern Agro-industry Technology Research System [nycytx-47]

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