一株具抗肿瘤活性的北极细菌的筛选及分子鉴定

在中国首次北极科学考察期间 ,从北极海域采集了水体 ,沉积物等样品 ,从中分离得到一批嗜冷细菌 ,采用MTT法对这些北极海洋细菌进行了细胞毒活性物质的筛选 ,得到一株具有细胞毒活性的菌株 .采用 16SrDNA序列分析及系统发育分析方法对该株菌株进行了分子鉴定 A number of psychrophilic microorganisms were isolated from arctic sea water and sediment samples collected during the China First Arctic Investigation cruise. A strain...

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Bibliographic Details
Main Authors: 曾润颖, 李根, 林昱
Format: Article in Journal/Newspaper
Language:Chinese
Published: 2002
Subjects:
北极细菌
MTT分析法
细胞毒活性物质
分子鉴定
arctic psychrophilic marine bacteria
MTT assay
molecular identification
Arctic
Online Access:http://dspace.xmu.edu.cn/handle/2288/141492
Description
Summary:在中国首次北极科学考察期间 ,从北极海域采集了水体 ,沉积物等样品 ,从中分离得到一批嗜冷细菌 ,采用MTT法对这些北极海洋细菌进行了细胞毒活性物质的筛选 ,得到一株具有细胞毒活性的菌株 .采用 16SrDNA序列分析及系统发育分析方法对该株菌株进行了分子鉴定 A number of psychrophilic microorganisms were isolated from arctic sea water and sediment samples collected during the China First Arctic Investigation cruise. A strain with cell toxicity was isolated by MTT screening model which was used to identify anti-tumor active material. The genera of the strain was identified based on comparison and phylogenetic analysis of 16S rDNA gene sequence. 中国首次北极科学考察研究课题;; 国家科技部基础性工作项目 (G 99A 15 )资助

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