Buffer-mediated activation of Candida antarctica lipase B dissolved in hydroxyl-functionalized ionic liquids

Ionic-liquid buffer having phosphate anion was synthesized for the development of buffered enzymatic ionic liquid systems Both the conformation and transesterification activity of Candida antarctica lipase B (CALB) dissolved in the hydroxyl-functionalized ionic liquids were buffer dependent Intrinsi...

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Bibliographic Details
Published in:Journal of Molecular Catalysis B: Enzymatic
Main Authors: Ou, Guangnan（Jimei Univ, Sch Bioengn）, Yang, Jing, He, Biyan（Jimei Univ, Sch Bioengn）, Yuan, Youzhu, 袁友珠
Format: Article in Journal/Newspaper
Language:English
Published: ELSEVIER SCIENCE BV 2010
Subjects:
Ionic-liquid buffer
Hydroxyl-functionalized ionic liquid
Lipase
Fluorescence
Antarc*
Antarctica
Online Access:https://doi.org/10.1016/j.molcatb.2010.09.013
http://dspace.xmu.edu.cn/handle/2288/11887
Description
Summary:Ionic-liquid buffer having phosphate anion was synthesized for the development of buffered enzymatic ionic liquid systems Both the conformation and transesterification activity of Candida antarctica lipase B (CALB) dissolved in the hydroxyl-functionalized ionic liquids were buffer dependent Intrinsic fluorescence studies indicated that the CALB possessed a more compact conformation in the medium consisted of ionic liquid buffer having phosphate anion and hydroxyl-functionalized ionic liquids like 1-(1-hydroxyethyl)-3-methyl-imidazolium tetrafluoroborate and 1-(1-hydroxyethyl)-3-methyl-imidazolium nitrate High activity and outstanding stability could be obtained with the CALB enzyme in the buffered ionic liquids for the transesterification (c) 2010 Elsevier B V All rights reserved NSFC[20873108, 20923004]; Science Foundation of Jimei University[2009CB939804]; Key Scientific Project of Fujian Province[2009HZ0002-1]

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