| Summary: | 以POlyI∶C(又称聚肌胞)为诱导剂,分别通过腹腔注射、浸泡和投喂3种途径诱导大菱鲆SCOPHTHAlMuS MAXIMuS体内抗病毒蛋白MX基因的转录,利用半定量rT-PCr方法检测干扰素下游基因-MX基因的转录水平来确定该诱导剂的诱导效果。结果显示,以上3种途径都能高效诱导MX蛋白MrnA的转录,均在48H之后达到高峰,其中以浸泡的方式更容易诱导MX基因转录,且在120H时仍保持较高水平。MX基因转录的时相变化证明了国产POlyI∶C可以通过多种途径诱导抗病毒蛋白MX的表达,为实际应用中确定用药途径提供了理论依据。另外,实验初步建立了半定量rT-PCr方法,为检测鱼体内干扰素的表达提供了技术方法。 Transcription of turbot Mx protein confirmed to be an antivirus factor was induced by PolyI∶C with intraperitoneal injection,immersion and oral administration.Mx mRNA was tested by semi-quantitative RT-PCR to validate the effect of PolyI∶C’s induction.The results illustrated that all the three induction ways could effectively induce the transcription of Mx gene and the peak of Mx mRNA quantity appeared at 48h after PolyI∶C application.The immersion method seemed to be the most effective way to induce Mx mRNA,as Mx mRNA could last more than 120h at a high level.This experiment proved that the homemade PolyI∶C could effectively induce Mx mRNA transcription in different applications,which provided a reference to use PolyI∶C in aquaculture practice. 国家863计划(2003AA622070);国家十一五科技支撑计划(2006BAD09A11);行业专项(nyhyzx07-046)共同资助
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