| Summary: | Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution February 2012 The distribution and magnitude of marine primary production helps determine the ocean's role in global carbon cycling. Constraining factors that impact this productivity and elucidating selective pressures that drive the composition of marine microbial communities are thus essential aspects of marine biogeochemistry. Vitamin B12, also known as cobalamin, is a cobalt containing organometallic micronutrient produced by some bacteria and archaea and required by many eukaryotic phytoplankton for methionine biosynthesis and regeneration. Although the potential for vitamin B12 availability to impact primary production and phytoplankton species composition has long been recognized, the lack of molecular-level tools for studying B12 production, use and acquisition has limited inquiry into the role of the vitamin in marine biogeochemical processes. This thesis describes the development of such tools and implements them for the study of B12 dynamics in an Antarctic shelf ecosystem. Nucleic acid probes for B12 biosynthesis genes were designed and used to identify a potentially dominant group of B12 producers in the Ross Sea. The activity of this group was then verified by mass spectrometry-based peptide measurements. Then, possible interconnections between iron and B12 dynamics in this region were identified using field-based bottle incubation experiments and vitamin uptake measurements, showing that iron availability may impact both B12 production and consumption. Changes in diatom proteomes induced by low B12 and low iron availability were then examined and used to identify a novel B12 acquisition protein, CBA 1, in diatoms. This represents the first identification of a B12 acquisition protein in eukaryotic phytoplankton. Transcripts encoding CBAl were detected in natural phytoplankton communities, confirming that B12 ...
|
|---|