Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry
© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Biogeosciences 15 (2018): 4923-4942, doi:10.5194/bg-15-4923-2018. Phaeocystis antarctica is an important phytoplankter of the Ross Sea where it domi...
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ftwhoas:oai:darchive.mblwhoilibrary.org:1912/10564 2023-05-15T13:48:31+02:00 Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry Bender, Sara J. Moran, Dawn M. McIlvin, Matthew R. Zheng, Hong McCrow, John P. Badger, Jonathan DiTullio, Giacomo R. Allen, Andrew E. Saito, Mak A. 2018-08-21 https://hdl.handle.net/1912/10564 en_US eng Copernicus Publications on behalf of the European Geosciences Union https://doi.org/10.5194/bg-15-4923-2018 Biogeosciences 15 (2018): 4923-4942 https://hdl.handle.net/1912/10564 doi:10.5194/bg-15-4923-2018 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ CC-BY Biogeosciences 15 (2018): 4923-4942 doi:10.5194/bg-15-4923-2018 Article 2018 ftwhoas https://doi.org/10.5194/bg-15-4923-2018 2022-05-28T23:00:28Z © The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Biogeosciences 15 (2018): 4923-4942, doi:10.5194/bg-15-4923-2018. Phaeocystis antarctica is an important phytoplankter of the Ross Sea where it dominates the early season bloom after sea ice retreat and is a major contributor to carbon export. The factors that influence Phaeocystis colony formation and the resultant Ross Sea bloom initiation have been of great scientific interest, yet there is little known about the underlying mechanisms responsible for these phenomena. Here, we present laboratory and field studies on Phaeocystis antarctica grown under multiple iron conditions using a coupled proteomic and transcriptomic approach. P. antarctica had a lower iron limitation threshold than a Ross Sea diatom Chaetoceros sp., and at increased iron nutrition (>120pM Fe') a shift from flagellate cells to a majority of colonial cells in P. antarctica was observed, implying a role for iron as a trigger for colony formation. Proteome analysis revealed an extensive and coordinated shift in proteome structure linked to iron availability and life cycle transitions with 327 and 436 proteins measured as significantly different between low and high iron in strains 1871 and 1374, respectively. The enzymes flavodoxin and plastocyanin that can functionally replace iron metalloenzymes were observed at low iron treatments consistent with cellular iron-sparing strategies, with plastocyanin having a larger dynamic range. The numerous isoforms of the putative iron-starvation-induced protein (ISIP) group (ISIP2A and ISIP3) had abundance patterns coinciding with that of either low or high iron (and coincident flagellate or the colonial cell types in strain 1871), implying that there may be specific iron acquisition systems for each life cycle type. The proteome analysis also revealed numerous structural proteins associated with each cell type: within flagellate cells actin and ... Article in Journal/Newspaper Antarc* Antarctica Ross Sea Sea ice Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) Ross Sea Biogeosciences 15 16 4923 4942 |
institution |
Open Polar |
collection |
Woods Hole Scientific Community: WHOAS (Woods Hole Open Access Server) |
op_collection_id |
ftwhoas |
language |
English |
description |
© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Biogeosciences 15 (2018): 4923-4942, doi:10.5194/bg-15-4923-2018. Phaeocystis antarctica is an important phytoplankter of the Ross Sea where it dominates the early season bloom after sea ice retreat and is a major contributor to carbon export. The factors that influence Phaeocystis colony formation and the resultant Ross Sea bloom initiation have been of great scientific interest, yet there is little known about the underlying mechanisms responsible for these phenomena. Here, we present laboratory and field studies on Phaeocystis antarctica grown under multiple iron conditions using a coupled proteomic and transcriptomic approach. P. antarctica had a lower iron limitation threshold than a Ross Sea diatom Chaetoceros sp., and at increased iron nutrition (>120pM Fe') a shift from flagellate cells to a majority of colonial cells in P. antarctica was observed, implying a role for iron as a trigger for colony formation. Proteome analysis revealed an extensive and coordinated shift in proteome structure linked to iron availability and life cycle transitions with 327 and 436 proteins measured as significantly different between low and high iron in strains 1871 and 1374, respectively. The enzymes flavodoxin and plastocyanin that can functionally replace iron metalloenzymes were observed at low iron treatments consistent with cellular iron-sparing strategies, with plastocyanin having a larger dynamic range. The numerous isoforms of the putative iron-starvation-induced protein (ISIP) group (ISIP2A and ISIP3) had abundance patterns coinciding with that of either low or high iron (and coincident flagellate or the colonial cell types in strain 1871), implying that there may be specific iron acquisition systems for each life cycle type. The proteome analysis also revealed numerous structural proteins associated with each cell type: within flagellate cells actin and ... |
format |
Article in Journal/Newspaper |
author |
Bender, Sara J. Moran, Dawn M. McIlvin, Matthew R. Zheng, Hong McCrow, John P. Badger, Jonathan DiTullio, Giacomo R. Allen, Andrew E. Saito, Mak A. |
spellingShingle |
Bender, Sara J. Moran, Dawn M. McIlvin, Matthew R. Zheng, Hong McCrow, John P. Badger, Jonathan DiTullio, Giacomo R. Allen, Andrew E. Saito, Mak A. Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
author_facet |
Bender, Sara J. Moran, Dawn M. McIlvin, Matthew R. Zheng, Hong McCrow, John P. Badger, Jonathan DiTullio, Giacomo R. Allen, Andrew E. Saito, Mak A. |
author_sort |
Bender, Sara J. |
title |
Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
title_short |
Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
title_full |
Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
title_fullStr |
Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
title_full_unstemmed |
Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
title_sort |
colony formation in phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
publisher |
Copernicus Publications on behalf of the European Geosciences Union |
publishDate |
2018 |
url |
https://hdl.handle.net/1912/10564 |
geographic |
Ross Sea |
geographic_facet |
Ross Sea |
genre |
Antarc* Antarctica Ross Sea Sea ice |
genre_facet |
Antarc* Antarctica Ross Sea Sea ice |
op_source |
Biogeosciences 15 (2018): 4923-4942 doi:10.5194/bg-15-4923-2018 |
op_relation |
https://doi.org/10.5194/bg-15-4923-2018 Biogeosciences 15 (2018): 4923-4942 https://hdl.handle.net/1912/10564 doi:10.5194/bg-15-4923-2018 |
op_rights |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.5194/bg-15-4923-2018 |
container_title |
Biogeosciences |
container_volume |
15 |
container_issue |
16 |
container_start_page |
4923 |
op_container_end_page |
4942 |
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1766249351037845504 |