Complement component C4-like protein in Atlantic cod (Gadus morhua L.) - Detection in ontogeny and identification of post-translational deimination in serum and extracellular vesicles
The complement system is a critical part of teleost immune defences, with complement component C4 forming part of the classical and lectin pathways. Cod C4-like protein was isolated from plasma, specific antibodies generated and C4-like protein was assessed in cod sera, mucus and in extracellular ve...
| Published in: | Developmental & Comparative Immunology |
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| Main Authors: | , , , |
| Format: | Article in Journal/Newspaper |
| Language: | unknown |
| Published: |
Elsevier
2019
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| Subjects: | |
| Online Access: | https://westminsterresearch.westminster.ac.uk/item/qvv59/complement-component-c4-like-protein-in-atlantic-cod-gadus-morhua-l-detection-in-ontogeny-and-identification-of-post-translational-deimination-in-serum-and-extracellular-vesicles https://westminsterresearch.westminster.ac.uk/download/7709bef90a88092137bb0a15c174534663cae1318dd794afed84280350f69a62/2392198/pagination_DCI_103437.pdf https://doi.org/10.1016/j.dci.2019.103437 |
| Summary: | The complement system is a critical part of teleost immune defences, with complement component C4 forming part of the classical and lectin pathways. Cod C4-like protein was isolated from plasma, specific antibodies generated and C4-like protein was assessed in cod sera, mucus and in extracellular vesicles (EVs) from serum and mucus. Higher levels of C4-like protein were detected in serum- than mucus-derived EVs. Post-translational deimination, caused by conversion of arginine into citrulline, can affect protein structure and function. Here we detected deiminated forms of C4-like protein in cod serum and at lower levels in mucus. C4-like protein was also found in deiminated form at low levels in EVs from both serum and mucus. C4-like protein was assessed by immunohistochemistry in cod larvae and detected in a range of organs including in liver, kidney, gut, muscle, skin and mucus, as well as in neuronal tissues of the brain, spinal cord and eye. This abundance of C4-like protein during early development may indicate roles in tissue remodelling, in addition to immune defences. The presence of deiminated C4-like protein in serum and mucosa, as well as in EVs, may suggest C4 protein moonlighting via post-translational deimination. |
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