Plant-made nervous necrosis virus-like particles protect fish against disease

Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-ass...

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Bibliographic Details
Published in:Frontiers in Plant Science
Main Authors: Marsian, Johanna, Hurdiss, Daniel L., Ranson, Neil A., Ritala, Anneli, Paley, Richard, Cano, Irene, Lomonossoff, George P.
Format: Article in Journal/Newspaper
Language:English
Published: 2019
Subjects:
Atlantic cod nervous necrosis virus
Protective immunity
Sea bass
Transient expression
Virus-like particles
/dk/atira/pure/sustainabledevelopmentgoals/life_below_water
name=SDG 14 - Life Below Water
atlantic cod
Online Access:https://cris.vtt.fi/en/publications/d960373c-3942-4aa0-8491-b9dc167ce39e
https://doi.org/10.3389/fpls.2019.00880
http://www.scopus.com/inward/record.url?scp=85069469768&partnerID=8YFLogxK
Description
Summary:Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.

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