| Summary: | Polyphasic analysis of four new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus , having 98 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on DNA gyrase subunit B ( gyrB ), RNA polymerase σ 70 factor ( rpoD ), replication origin-binding protein ( rctB ) and transmembrane regulatory protein ( toxR ) genes, fluorescent amplified fragment length polymorphism and DNA–DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed that these new isolates should be accommodated in a novel species, Vibrio gigantis sp. nov. Phenotypic features that differentiate V. gigantis from other known Vibrio species include arginine dihydrolase, gelatinase and β -galactosidase activities, NO 2 production, growth at 35 °C, and utilization of sucrose, melibiose, amygdalin, glycerol, galactose, starch and glycogen. The type strain is LGP 13 T (=LMG 22741 T =CIP 108656 T ).
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