An oral challenge for turbot larvae with Vibrio anguillarum

• Main Authors: Chair, M., Dehasque, M., Van Poucke, S., Nelis, H., Sorgeloos, P., De Leenheer, A.P.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 1994
• Subjects: Bacterial diseases; Digestive system; Disease control; Fish culture; Fish diseases; Fish larvae; Marine fish; Pathogenic bacteria; Vibrio anguillarum; Scophthalmus maximus; Turbot
• Online Access: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2552

There is a need for a standard in vitro procedure to infect larval fish with bacteria. Such a procedure could be used as a tool to evaluate fish quality, verify fish sensitivity to a certain bacterial strain or evaluate the pathogenicity of a bacterium towards a given fish species. The availability of a challenge test is a prerequisite in studies dealing with the effect of different medicinal treatments on diseased fish. An oral challenge test would offer the possibility to investigate the routes of infection via the digestive tract, i.e. after feeding contaminated feeds. The present study aims to verify this hypothesis and proposes a standard challenge procedure for turbot ( Scophthalmus maximus ) larvae with a pathogenic Vibrio anguillarum strain.