Summary: | Water soluble sarcoplasmic proteins of nine Gadoid species were chromatographed using a C 4 wide pore reversed phase column and gradient elution with trifluoracetic acid, water and acetonitrile. In most cases, nine peaks could be identified in cod ( Gadus morhua ), with four major peaks always present. Direct visual comparison of unknown chromatograms with a cod chromatogram kept in memory by the data processor allowed the elimination of most non-cod Gadoids. Appearance of peaks not found in cod confirmed this elimination. In case of doubt, however, confirmation by electrophoretic technics is recommended. HPLC appeared to be a quick screening technique for identifying cod by eliminating non-cod species.
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