Identification of cod fillets by HPLC: a screening method

Water soluble sarcoplasmic proteins of nine Gadoid species were chromatographed using a C 4 wide pore reversed phase column and gradient elution with trifluoracetic acid, water and acetonitrile. In most cases, nine peaks could be identified in cod ( Gadus morhua ), with four major peaks always prese...

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Bibliographic Details
Main Author: Vyncke, W.
Format: Book
Language:English
Published: 1992
Subjects:
Online Access:https://www.vliz.be/imisdocs/publications/257775.pdf
Description
Summary:Water soluble sarcoplasmic proteins of nine Gadoid species were chromatographed using a C 4 wide pore reversed phase column and gradient elution with trifluoracetic acid, water and acetonitrile. In most cases, nine peaks could be identified in cod ( Gadus morhua ), with four major peaks always present. Direct visual comparison of unknown chromatograms with a cod chromatogram kept in memory by the data processor allowed the elimination of most non-cod Gadoids. Appearance of peaks not found in cod confirmed this elimination. In case of doubt, however, confirmation by electrophoretic technics is recommended. HPLC appeared to be a quick screening technique for identifying cod by eliminating non-cod species.