Clostridium botulinum toxin development in refrigerated reduced oxygen packaged Atlantic croaker (Micropogonias undulatus)
The purpose of this study was to determine the effects of storage temperature and film oxygen transmission rate (OTR) on toxin development by Clostridium botulinum in refrigerated raw vacuum packaged croaker fillets, and to determine if toxin development precedes microbiological and/or organoleptic...
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Virginia Tech
2007
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ftvirginiatec:oai:vtechworks.lib.vt.edu:10919/32440 2024-05-19T07:38:16+00:00 Clostridium botulinum toxin development in refrigerated reduced oxygen packaged Atlantic croaker (Micropogonias undulatus) Rheinhart, Courtney Elizabeth Food Science and Technology Eifert, Joseph D. Sumner, Susan S. Jahncke, Michael L. 2007-04-30 application/pdf http://hdl.handle.net/10919/32440 http://scholar.lib.vt.edu/theses/available/etd-05092007-160646/ unknown Virginia Tech THESISFINAL3.pdf etd-05092007-160646 http://hdl.handle.net/10919/32440 http://scholar.lib.vt.edu/theses/available/etd-05092007-160646/ In Copyright http://rightsstatements.org/vocab/InC/1.0/ C. botulinum croaker spoilage vacuum packaging Thesis 2007 ftvirginiatec 2024-05-01T01:17:18Z The purpose of this study was to determine the effects of storage temperature and film oxygen transmission rate (OTR) on toxin development by Clostridium botulinum in refrigerated raw vacuum packaged croaker fillets, and to determine if toxin development precedes microbiological and/or organoleptic spoilage. Raw croaker fillets were vacuum packaged in oxygen-permeable films (OTR of 10,000 cc/m2/24hr or 3,000 cc/m2/24hr) and stored at either 4ºC or 10ºC. Type 83F, 17 Type B, Beluga, Minnesota, and Alaska nonproteolytic strains of C. botulinum were used to inoculate fish prior to vacuum packaging. At both temperatures, microbial spoilage preceded toxin production in fillets vacuum packaged in both film types. At 4ºC microbial spoilage occurred after approximately 7 days for fillets vacuum packaged in the 10,000 cc/m2/24hr OTR film and after 8 days for fillets vacuum packaged in the 3,000 cc/m2/24hr OTR film. However, toxin was not detected until day 8. At 10ºC microbial spoilage occurred after approximately 3 days for fillets vacuum packaged in the 10,000 cc/m2/24hr OTR film, while toxin production occurred on day 5. For fillets vacuum packaged in the 3,000 cc/m2/24hr OTR film microbial spoilage occurred after 4 days. However toxin production did not occur until day 6. In contrast, at both temperatures toxin production preceded or coincided with organoleptic spoilage in fillets vacuum packaged in both film types. At 4ºC organoleptic spoilage occurred after 10 days for fillets packaged in the 10,000 cc/m2/24hr OTR film and after 9 days in the 3,000 cc/m2/24hr OTR film, while toxin production occurred on day 8. At 10ºC organoleptic spoilage occurred after 6 days for fillets packaged in the 10,000 cc/m2/24hr OTR film, and toxin was detected on day 5. For fillets packaged in the 3,000 cc/m2/24hr OTR film and stored at 10ºC, organoleptic spoilage occurred after 6 days, while toxin production occurred on day 6. Although toxin production preceded or coincided with organoleptic spoilage in both film types, this may have ... Thesis Beluga Beluga* Alaska VTechWorks (VirginiaTech) |
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Open Polar |
collection |
VTechWorks (VirginiaTech) |
op_collection_id |
ftvirginiatec |
language |
unknown |
topic |
C. botulinum croaker spoilage vacuum packaging |
spellingShingle |
C. botulinum croaker spoilage vacuum packaging Rheinhart, Courtney Elizabeth Clostridium botulinum toxin development in refrigerated reduced oxygen packaged Atlantic croaker (Micropogonias undulatus) |
topic_facet |
C. botulinum croaker spoilage vacuum packaging |
description |
The purpose of this study was to determine the effects of storage temperature and film oxygen transmission rate (OTR) on toxin development by Clostridium botulinum in refrigerated raw vacuum packaged croaker fillets, and to determine if toxin development precedes microbiological and/or organoleptic spoilage. Raw croaker fillets were vacuum packaged in oxygen-permeable films (OTR of 10,000 cc/m2/24hr or 3,000 cc/m2/24hr) and stored at either 4ºC or 10ºC. Type 83F, 17 Type B, Beluga, Minnesota, and Alaska nonproteolytic strains of C. botulinum were used to inoculate fish prior to vacuum packaging. At both temperatures, microbial spoilage preceded toxin production in fillets vacuum packaged in both film types. At 4ºC microbial spoilage occurred after approximately 7 days for fillets vacuum packaged in the 10,000 cc/m2/24hr OTR film and after 8 days for fillets vacuum packaged in the 3,000 cc/m2/24hr OTR film. However, toxin was not detected until day 8. At 10ºC microbial spoilage occurred after approximately 3 days for fillets vacuum packaged in the 10,000 cc/m2/24hr OTR film, while toxin production occurred on day 5. For fillets vacuum packaged in the 3,000 cc/m2/24hr OTR film microbial spoilage occurred after 4 days. However toxin production did not occur until day 6. In contrast, at both temperatures toxin production preceded or coincided with organoleptic spoilage in fillets vacuum packaged in both film types. At 4ºC organoleptic spoilage occurred after 10 days for fillets packaged in the 10,000 cc/m2/24hr OTR film and after 9 days in the 3,000 cc/m2/24hr OTR film, while toxin production occurred on day 8. At 10ºC organoleptic spoilage occurred after 6 days for fillets packaged in the 10,000 cc/m2/24hr OTR film, and toxin was detected on day 5. For fillets packaged in the 3,000 cc/m2/24hr OTR film and stored at 10ºC, organoleptic spoilage occurred after 6 days, while toxin production occurred on day 6. Although toxin production preceded or coincided with organoleptic spoilage in both film types, this may have ... |
author2 |
Food Science and Technology Eifert, Joseph D. Sumner, Susan S. Jahncke, Michael L. |
format |
Thesis |
author |
Rheinhart, Courtney Elizabeth |
author_facet |
Rheinhart, Courtney Elizabeth |
author_sort |
Rheinhart, Courtney Elizabeth |
title |
Clostridium botulinum toxin development in refrigerated reduced oxygen packaged Atlantic croaker (Micropogonias undulatus) |
title_short |
Clostridium botulinum toxin development in refrigerated reduced oxygen packaged Atlantic croaker (Micropogonias undulatus) |
title_full |
Clostridium botulinum toxin development in refrigerated reduced oxygen packaged Atlantic croaker (Micropogonias undulatus) |
title_fullStr |
Clostridium botulinum toxin development in refrigerated reduced oxygen packaged Atlantic croaker (Micropogonias undulatus) |
title_full_unstemmed |
Clostridium botulinum toxin development in refrigerated reduced oxygen packaged Atlantic croaker (Micropogonias undulatus) |
title_sort |
clostridium botulinum toxin development in refrigerated reduced oxygen packaged atlantic croaker (micropogonias undulatus) |
publisher |
Virginia Tech |
publishDate |
2007 |
url |
http://hdl.handle.net/10919/32440 http://scholar.lib.vt.edu/theses/available/etd-05092007-160646/ |
genre |
Beluga Beluga* Alaska |
genre_facet |
Beluga Beluga* Alaska |
op_relation |
THESISFINAL3.pdf etd-05092007-160646 http://hdl.handle.net/10919/32440 http://scholar.lib.vt.edu/theses/available/etd-05092007-160646/ |
op_rights |
In Copyright http://rightsstatements.org/vocab/InC/1.0/ |
_version_ |
1799477684570423296 |