SEQUENCE OF THE STREPTOMYCES-ALBUS-G LIPASE-ENCODING GENE REVEALS THE PRESENCE OF A PROKARYOTIC LIPASE FAMILY
An extracellular lipase (Lip)-encoding gene from Streptomyces albus G has been cloned and sequenced. It encodes a Lip with 82% sequence identity to another previously cloned Lip from a Streptomyces species not closely related. These two sequences can be aligned with 33% identity to the sequence of L...
| Format: | Other Non-Article Part of Journal/Newspaper |
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| Language: | unknown |
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ELSEVIER SCIENCE BV
1994
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| Online Access: | http://wrap.warwick.ac.uk/20554/ |
| Summary: | An extracellular lipase (Lip)-encoding gene from Streptomyces albus G has been cloned and sequenced. It encodes a Lip with 82% sequence identity to another previously cloned Lip from a Streptomyces species not closely related. These two sequences can be aligned with 33% identity to the sequence of Lip1 from the antarctic psychrotroph Moraxella TA144 [G. Feller et al., Nucleic Acids Res. 18 (1990) 6431]. An alignment of the three sequences revealed amino-acid substitutions which might be responsible for the greater thermal stability of the Streptomyces lipases. The presence of this lip gene family in several members of the Streptomyces genus was also shown. |
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