Mass spectrometry data from a quantitative analysis of protein expression in gills of immuno-challenged blue mussels (Mytilus edulis)

• Published in: Data in Brief
• Main Authors: Hörnaeus, Katarina, Guillemant, Julie, Mi, Jia, Hernroth, Bodil, Bergquist, Jonas, Bergström Lind, Sara
• Format: Article in Journal/Newspaper
• Language: English
• Published: Uppsala universitet, Analytisk kemi 2016
• Subjects: Mass spectrometry data; Blue mussels; Mytilus edulis; Gills; Quantitative proteomics; Analytical Chemistry; Analytisk kemi; Ocean acidification
• Online Access: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-301959; https://doi.org/10.1016/j.dib.2016.05.073

Here, we provide the dataset associated with our research article on the potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, "Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis)" [1]. Blue mussels were stimulated with lipopolysaccharides and samples were collected at different time points post injection. Protein extracts were prepared from the gills, digested using trypsin and a full in-depth proteome investigation was performed using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Protein identification and quantification was performed using the MaxQuant 1.5.1.2 software, "MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification" [2].