Mass spectrometry data from a quantitative analysis of protein expression in gills of immuno-challenged blue mussels (Mytilus edulis)

Here, we provide the dataset associated with our research article on the potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, "Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis)" [...

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Bibliographic Details
Published in:Data in Brief
Main Authors: Hörnaeus, Katarina, Guillemant, Julie, Mi, Jia, Hernroth, Bodil, Bergquist, Jonas, Bergström Lind, Sara
Format: Article in Journal/Newspaper
Language:English
Published: Uppsala universitet, Analytisk kemi 2016
Subjects:
Mass spectrometry data
Blue mussels
Mytilus edulis
Gills
Quantitative proteomics
Analytical Chemistry
Analytisk kemi
Ocean acidification
Online Access:http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-301959
https://doi.org/10.1016/j.dib.2016.05.073
Description
Summary:Here, we provide the dataset associated with our research article on the potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, "Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis)" [1]. Blue mussels were stimulated with lipopolysaccharides and samples were collected at different time points post injection. Protein extracts were prepared from the gills, digested using trypsin and a full in-depth proteome investigation was performed using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Protein identification and quantification was performed using the MaxQuant 1.5.1.2 software, "MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification" [2].

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