Molecular and cytogenetic characterization of repetitive DNA in the Antarctic polyplacophoran Nuttallochiton mirandus

• Published in: Chromosome Research
• Main Authors: BISCOTTI, MARIA ASSUNTA, BARUCCA, MARCO, OLMO, ETTORE, CANAPA, Adriana, CAPRIGLIONE T, ODIERNA G
• Other Authors: Biscotti, MARIA ASSUNTA, Barucca, Marco, Capriglione, T, Odierna, G, Olmo, Ettore, Canapa, Adriana
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2008
• Subjects: Antarc*; Antarctic
• Online Access: http://hdl.handle.net/11566/51961; https://doi.org/10.1007/s10577-008-1248-0

Two highly repeated DNAs, designated NmE1/NmE2 and NmE5, were identified by EcoRV digestion in the chiton Nuttallochiton mirandus (Mollusca: Polyplacophora). The comparison of the sequences obtained showed high similarity in 5' and 3' regions and the NmE5 sequence displayed an inserted sequence that might arise from a transposable element. Southern blotting analyses suggested a tandem organization of both satellite DNA families identified. Moreover, dot blot analyses, performed on several molluscan species, revealed a different degree of conservation of the repeated DNAs. Fluorescence in-situ hybridizations (FISH) on metaphase chromosomes showed that both satellite DNAs are located at centromeric regions.