Molecular and cytogenetic characterization of repetitive DNA in the Antarctic polyplacophoran Nuttallochiton mirandus

Two highly repeated DNAs, designated NmE1/NmE2 and NmE5, were identified by EcoRV digestion in the chiton Nuttallochiton mirandus (Mollusca: Polyplacophora). The comparison of the sequences obtained showed high similarity in 5' and 3' regions and the NmE5 sequence displayed an inserted seq...

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Bibliographic Details
Published in:Chromosome Research
Main Authors: BISCOTTI, MARIA ASSUNTA, BARUCCA, MARCO, OLMO, ETTORE, CANAPA, Adriana, CAPRIGLIONE T, ODIERNA G
Other Authors: Biscotti, MARIA ASSUNTA, Barucca, Marco, Capriglione, T, Odierna, G, Olmo, Ettore, Canapa, Adriana
Format: Article in Journal/Newspaper
Language:English
Published: 2008
Subjects:
Antarc*
Antarctic
Online Access:http://hdl.handle.net/11566/51961
https://doi.org/10.1007/s10577-008-1248-0
Description
Summary:Two highly repeated DNAs, designated NmE1/NmE2 and NmE5, were identified by EcoRV digestion in the chiton Nuttallochiton mirandus (Mollusca: Polyplacophora). The comparison of the sequences obtained showed high similarity in 5' and 3' regions and the NmE5 sequence displayed an inserted sequence that might arise from a transposable element. Southern blotting analyses suggested a tandem organization of both satellite DNA families identified. Moreover, dot blot analyses, performed on several molluscan species, revealed a different degree of conservation of the repeated DNAs. Fluorescence in-situ hybridizations (FISH) on metaphase chromosomes showed that both satellite DNAs are located at centromeric regions.

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