Expression of Na+, K+-ATPase B subunit isoforms in the European Eel (Anguilla anguilla)

Reverse transcriptase - polymerase chain reaction experiments utilising degenerate primers were used to demonstrate that the Na+,K+-ATPase beta 3 but not beta 2 isoform was present in brain tissue from the European eel (Anguilla anguilla). Two cDNA fragments were cloned and sequenced yielding 445 nu...

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Bibliographic Details
Main Authors: Cramb, Gordon, Cutler, Christopher Paul, Sanders, IL
Format: Article in Journal/Newspaper
Language:English
Published: 1997
Subjects:
Anguilla anguilla
beta 2 isoform
beta 3 isoform
brain
cDNA
eel
mRNA
Na+,K+-ATPase
nucleotide sequence
PCR
PRIMARY SEQUENCE
FUNCTIONAL EXPRESSION
TISSUE-SPECIFICITY
XENOPUS-LAEVIS
NA,K-ATPASE
TRANSPORT
SEAWATER
ATPASE
CELLS
Online Access:https://risweb.st-andrews.ac.uk/portal/en/researchoutput/expression-of-na-katpase-b-subunit-isoforms-in-the-european-eel-anguilla-anguilla(cf36fa8b-a9ae-4a2c-8593-fbcaca8c5f48).html
http://www.scopus.com/inward/record.url?scp=0000812543&partnerID=8YFLogxK
http://www.wkap.nl./journalhome.htm/0920-1742
Description
Summary:Reverse transcriptase - polymerase chain reaction experiments utilising degenerate primers were used to demonstrate that the Na+,K+-ATPase beta 3 but not beta 2 isoform was present in brain tissue from the European eel (Anguilla anguilla). Two cDNA fragments were cloned and sequenced yielding 445 nucleotides of the Na+,K+-ATPase beta 3 isoform sequence. The putative amino acid sequence translated from this nucleotide sequence shared 58.0, 50.6 and 50.3% homology with previously published Na+,K+-ATPase "beta 3-like" isoform sequences from the clawed toad (Xenopus laevis), the chicken (Gallus domesticus) and the toad (Bufo marinus) respectively. The size of the Na+,K+-ATPase beta 3 mRNA transcript in eel tissues was demonstrated to be 2.4 Kb. Detectable levels of Na+,K+-ATPase beta 3 mRNA were only found in brain tissue. The tissue specific distribution of the beta 3 mRNA suggests that it plays little or no direct role in the process of osmoregulation in tissues such as the intestine or gill.

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