Expression of a duplicate Na, K-ATPase B1 isoform in the European eel (Anguilla anguilla)

Recent studies on teleost fish have suggested that their genomes have undergone ancient polyploidization events resulting in the duplication of the genome. A duplicate copy of the Na,K-ATPase beta(1)-isoform (called beta(233)) has been identified in the European eel (Anguilla anguilla). The beta(233...

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Bibliographic Details
Main Authors: Cutler, Christopher Paul, Brezillon, Stephane, Bekir, S, Sanders, Ian L, Hazon, Neil, Cramb, Gordon
Format: Article in Journal/Newspaper
Language:English
Published: 2000
Subjects:
teleost fish
mRNA expression
salinity acclimation
developmental maturation
immunolocalization
BETA-SUBUNIT
TISSUE-SPECIFICITY
PRIMARY SEQUENCE
ALPHA-SUBUNIT
CHLORIDE CELL
CIS-ELEMENTS
NA+,K+-ATPASE
GENE
ATPASE
RNA
Anguilla anguilla
Online Access:https://risweb.st-andrews.ac.uk/portal/en/researchoutput/expression-of-a-duplicate-na-katpase-b1-isoform-in-the-european-eel-anguilla-anguilla(1f66f9c7-f4f7-4812-9f46-4938df83735f).html
http://www.ajpregu.org
Description
Summary:Recent studies on teleost fish have suggested that their genomes have undergone ancient polyploidization events resulting in the duplication of the genome. A duplicate copy of the Na,K-ATPase beta(1)-isoform (called beta(233)) has been identified in the European eel (Anguilla anguilla). The beta(233)-isoform shares high levels of nucleotide (74.8%) and amino acid (69.9%) homology with the eel beta(1)-subunit as well as other vertebrate beta(1)-sequences. Compared with the widely expressed beta(1)-isoform, expression of beta(233)-mRNA is mainly restricted to epithelial tissues. Seawater acclimation induced increases in beta(233)-mRNA levels in kidney, gill, and intestine of migratory "silver" but not the nonmigratory "yellow" adult eels, suggesting that the factors responsible for this upregulation are themselves developmentally regulated. Expression of a variably glycosylated 40- to 52-kDa beta(233)-protein in both gill "chloride" and intestinal epithelial cells suggests that the beta(233)-isoform of Na,K-ATPase may play an important functional role in the major osmoregulatory tissues of euryhaline fish such as the eel.

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