Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr ( Salvelinus alpinus ).
Arctic charr (Salvelinus alpinus L.) from Lake Thingvallavatn, Iceland occur as four distinct morphs: large benthivorous (LB), dwarf benthivorous (DB), piscivorous (PI) and planktonivorous (PL). The morphs differ with respect to body size, head morphology, growth rate, and life history. The aim of t...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2006
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Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/characterisation-and-expression-of-the-paired-box-protein-7-pax7-gene-in-polymorphic-arctic-charr-salvelinus-alpinus(fc35edb6-aefc-4a04-b037-8832f14f379e).html https://doi.org/10.1016/j.cbpb.2006.08.013 http://www.scopus.com/inward/record.url?scp=33845202335&partnerID=8YFLogxK |
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ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/fc35edb6-aefc-4a04-b037-8832f14f379e 2024-06-23T07:48:25+00:00 Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr ( Salvelinus alpinus ). Sibthorpe, D Sturlaugsdottir, ~Missing Data Kristjansson, B J Thorarensen, H Skulason, S Johnston, Ian Alistair 2006-11 https://research-portal.st-andrews.ac.uk/en/researchoutput/characterisation-and-expression-of-the-paired-box-protein-7-pax7-gene-in-polymorphic-arctic-charr-salvelinus-alpinus(fc35edb6-aefc-4a04-b037-8832f14f379e).html https://doi.org/10.1016/j.cbpb.2006.08.013 http://www.scopus.com/inward/record.url?scp=33845202335&partnerID=8YFLogxK eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/characterisation-and-expression-of-the-paired-box-protein-7-pax7-gene-in-polymorphic-arctic-charr-salvelinus-alpinus(fc35edb6-aefc-4a04-b037-8832f14f379e).html info:eu-repo/semantics/restrictedAccess Sibthorpe , D , Sturlaugsdottir , M D , Kristjansson , B J , Thorarensen , H , Skulason , S & Johnston , I A 2006 , ' Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr ( Salvelinus alpinus ). ' , Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology , vol. 145 , pp. 371-383 . https://doi.org/10.1016/j.cbpb.2006.08.013 salmonid Arctic chart Salvelinus alpinus resource polymorphism morph pax7 multiple splice variants evolution skeletal muscle development TROUT ONCORHYNCHUS-MYKISS 4 SYMPATRIC MORPHS SKELETAL-MUSCLE ADULT MICE INHERITED DIFFERENCES GENOME DUPLICATION MESSENGER-RNAS KINASE CK2 DOMAIN TRANSCRIPTS article 2006 ftunstandrewcris https://doi.org/10.1016/j.cbpb.2006.08.013 2024-06-13T00:18:44Z Arctic charr (Salvelinus alpinus L.) from Lake Thingvallavatn, Iceland occur as four distinct morphs: large benthivorous (LB), dwarf benthivorous (DB), piscivorous (PI) and planktonivorous (PL). The morphs differ with respect to body size, head morphology, growth rate, and life history. The aim of this study was to investigate the paired box protein 7 (Pax7) gene as a candidate for such polymorphisms due to its importance in cranio-facial, skeletal muscle, and central nervous system development. No variation in coding and intronic sequences was found between morphs. We identified 10 alternate Pax7 isoforms with insertions/deletions: a four-residue (GNRT) deletion, a GEASS insertion truncated by the first serine residue (GEAS), and a thirteen-residue insertion (GQYA/TGPEYVYCGT). The latter insertion with a threonine (T) contains a putative casein kinase II (CK-2) phosphorylation site. Pax7 spatial expression patterns were identical in embryos of DB-, LB-, and PL-morphs, and were similar to those described for zebrafish Pax7c, but a difference in temporal expression for segmentation was observed between DB and LB morphs. At the end of segmentation, novel expression was observed in the mandibular region as two bilateral domains. The potential role of multiple alternative splicing of the Pax7 gene for the generation of different Arctic charr morphs is briefly discussed. (c) 2006 Elsevier Inc. All rights reserved. Article in Journal/Newspaper Arctic Arctic charr Arctic Iceland Salvelinus alpinus University of St Andrews: Research Portal Arctic Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 145 3-4 371 383 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
salmonid Arctic chart Salvelinus alpinus resource polymorphism morph pax7 multiple splice variants evolution skeletal muscle development TROUT ONCORHYNCHUS-MYKISS 4 SYMPATRIC MORPHS SKELETAL-MUSCLE ADULT MICE INHERITED DIFFERENCES GENOME DUPLICATION MESSENGER-RNAS KINASE CK2 DOMAIN TRANSCRIPTS |
spellingShingle |
salmonid Arctic chart Salvelinus alpinus resource polymorphism morph pax7 multiple splice variants evolution skeletal muscle development TROUT ONCORHYNCHUS-MYKISS 4 SYMPATRIC MORPHS SKELETAL-MUSCLE ADULT MICE INHERITED DIFFERENCES GENOME DUPLICATION MESSENGER-RNAS KINASE CK2 DOMAIN TRANSCRIPTS Sibthorpe, D Sturlaugsdottir, ~Missing Data Kristjansson, B J Thorarensen, H Skulason, S Johnston, Ian Alistair Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr ( Salvelinus alpinus ). |
topic_facet |
salmonid Arctic chart Salvelinus alpinus resource polymorphism morph pax7 multiple splice variants evolution skeletal muscle development TROUT ONCORHYNCHUS-MYKISS 4 SYMPATRIC MORPHS SKELETAL-MUSCLE ADULT MICE INHERITED DIFFERENCES GENOME DUPLICATION MESSENGER-RNAS KINASE CK2 DOMAIN TRANSCRIPTS |
description |
Arctic charr (Salvelinus alpinus L.) from Lake Thingvallavatn, Iceland occur as four distinct morphs: large benthivorous (LB), dwarf benthivorous (DB), piscivorous (PI) and planktonivorous (PL). The morphs differ with respect to body size, head morphology, growth rate, and life history. The aim of this study was to investigate the paired box protein 7 (Pax7) gene as a candidate for such polymorphisms due to its importance in cranio-facial, skeletal muscle, and central nervous system development. No variation in coding and intronic sequences was found between morphs. We identified 10 alternate Pax7 isoforms with insertions/deletions: a four-residue (GNRT) deletion, a GEASS insertion truncated by the first serine residue (GEAS), and a thirteen-residue insertion (GQYA/TGPEYVYCGT). The latter insertion with a threonine (T) contains a putative casein kinase II (CK-2) phosphorylation site. Pax7 spatial expression patterns were identical in embryos of DB-, LB-, and PL-morphs, and were similar to those described for zebrafish Pax7c, but a difference in temporal expression for segmentation was observed between DB and LB morphs. At the end of segmentation, novel expression was observed in the mandibular region as two bilateral domains. The potential role of multiple alternative splicing of the Pax7 gene for the generation of different Arctic charr morphs is briefly discussed. (c) 2006 Elsevier Inc. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Sibthorpe, D Sturlaugsdottir, ~Missing Data Kristjansson, B J Thorarensen, H Skulason, S Johnston, Ian Alistair |
author_facet |
Sibthorpe, D Sturlaugsdottir, ~Missing Data Kristjansson, B J Thorarensen, H Skulason, S Johnston, Ian Alistair |
author_sort |
Sibthorpe, D |
title |
Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr ( Salvelinus alpinus ). |
title_short |
Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr ( Salvelinus alpinus ). |
title_full |
Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr ( Salvelinus alpinus ). |
title_fullStr |
Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr ( Salvelinus alpinus ). |
title_full_unstemmed |
Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr ( Salvelinus alpinus ). |
title_sort |
characterisation and expression of the paired box protein 7 (pax7) gene in polymorphic arctic charr ( salvelinus alpinus ). |
publishDate |
2006 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/characterisation-and-expression-of-the-paired-box-protein-7-pax7-gene-in-polymorphic-arctic-charr-salvelinus-alpinus(fc35edb6-aefc-4a04-b037-8832f14f379e).html https://doi.org/10.1016/j.cbpb.2006.08.013 http://www.scopus.com/inward/record.url?scp=33845202335&partnerID=8YFLogxK |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic charr Arctic Iceland Salvelinus alpinus |
genre_facet |
Arctic Arctic charr Arctic Iceland Salvelinus alpinus |
op_source |
Sibthorpe , D , Sturlaugsdottir , M D , Kristjansson , B J , Thorarensen , H , Skulason , S & Johnston , I A 2006 , ' Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr ( Salvelinus alpinus ). ' , Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology , vol. 145 , pp. 371-383 . https://doi.org/10.1016/j.cbpb.2006.08.013 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/characterisation-and-expression-of-the-paired-box-protein-7-pax7-gene-in-polymorphic-arctic-charr-salvelinus-alpinus(fc35edb6-aefc-4a04-b037-8832f14f379e).html |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.cbpb.2006.08.013 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
145 |
container_issue |
3-4 |
container_start_page |
371 |
op_container_end_page |
383 |
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1802638834436931584 |