Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr ( Salvelinus alpinus ).

Arctic charr (Salvelinus alpinus L.) from Lake Thingvallavatn, Iceland occur as four distinct morphs: large benthivorous (LB), dwarf benthivorous (DB), piscivorous (PI) and planktonivorous (PL). The morphs differ with respect to body size, head morphology, growth rate, and life history. The aim of t...

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Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Main Authors: Sibthorpe, D, Sturlaugsdottir, ~Missing Data, Kristjansson, B J, Thorarensen, H, Skulason, S, Johnston, Ian Alistair
Format: Article in Journal/Newspaper
Language:English
Published: 2006
Subjects:
salmonid
Arctic chart
Salvelinus alpinus
resource polymorphism
morph
pax7
multiple splice variants
evolution
skeletal muscle
development
TROUT ONCORHYNCHUS-MYKISS
4 SYMPATRIC MORPHS
SKELETAL-MUSCLE
ADULT MICE
INHERITED DIFFERENCES
GENOME DUPLICATION
MESSENGER-RNAS
KINASE CK2
DOMAIN
TRANSCRIPTS
Arctic
Arctic charr
Iceland
Online Access:https://research-portal.st-andrews.ac.uk/en/researchoutput/characterisation-and-expression-of-the-paired-box-protein-7-pax7-gene-in-polymorphic-arctic-charr-salvelinus-alpinus(fc35edb6-aefc-4a04-b037-8832f14f379e).html
https://doi.org/10.1016/j.cbpb.2006.08.013
http://www.scopus.com/inward/record.url?scp=33845202335&partnerID=8YFLogxK
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Summary:Arctic charr (Salvelinus alpinus L.) from Lake Thingvallavatn, Iceland occur as four distinct morphs: large benthivorous (LB), dwarf benthivorous (DB), piscivorous (PI) and planktonivorous (PL). The morphs differ with respect to body size, head morphology, growth rate, and life history. The aim of this study was to investigate the paired box protein 7 (Pax7) gene as a candidate for such polymorphisms due to its importance in cranio-facial, skeletal muscle, and central nervous system development. No variation in coding and intronic sequences was found between morphs. We identified 10 alternate Pax7 isoforms with insertions/deletions: a four-residue (GNRT) deletion, a GEASS insertion truncated by the first serine residue (GEAS), and a thirteen-residue insertion (GQYA/TGPEYVYCGT). The latter insertion with a threonine (T) contains a putative casein kinase II (CK-2) phosphorylation site. Pax7 spatial expression patterns were identical in embryos of DB-, LB-, and PL-morphs, and were similar to those described for zebrafish Pax7c, but a difference in temporal expression for segmentation was observed between DB and LB morphs. At the end of segmentation, novel expression was observed in the mandibular region as two bilateral domains. The potential role of multiple alternative splicing of the Pax7 gene for the generation of different Arctic charr morphs is briefly discussed. (c) 2006 Elsevier Inc. All rights reserved.

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